Plackett Timothy P, Kovacs Elizabeth J
Burn and Shock Trauma Institute, Departments of Surgery, Cell Biology, Neurobiology,and Anatomy, Alcohol Research Program, Loyola University Medical Center, Maywood, IL, USA.
Methods Mol Biol. 2008;447:3-9. doi: 10.1007/978-1-59745-242-7_1.
Acute alcohol administration has minimal effects on basal immune function. However, when the immune system is challenged, acute alcohol administration alters the immune system's response. In the first 3 h after infection or traumatic injury, the presence of alcohol is associated with a decreased inflammatory response. This defect lasts long after the alcohol is cleared. Conversely, by 48 h after traumatic injury, the presence of alcohol is associated with a heightened inflammatory response. Aside from its in vivo actions, systemic administration of alcohol also alters the ex vivo response of immune cells, resulting in a decreased production of multiple cytokines after stimulation by lipopolysaccharide, concanavilin A, zymosan, and CpG DNA. Here, we describe a standardized model of acute administration of ethanol to mice used to study both the in vivo and ex vivo responses of immune cells to ethanol.
急性酒精摄入对基础免疫功能影响极小。然而，当免疫系统受到挑战时，急性酒精摄入会改变免疫系统的反应。在感染或创伤性损伤后的最初3小时内，酒精的存在与炎症反应减弱有关。这种缺陷在酒精清除后仍会持续很长时间。相反，在创伤性损伤后48小时，酒精的存在与炎症反应增强有关。除了其体内作用外，酒精的全身给药还会改变免疫细胞的体外反应，导致在受到脂多糖、伴刀豆球蛋白A、酵母聚糖和CpG DNA刺激后多种细胞因子的产生减少。在此，我们描述了一种向小鼠急性给予乙醇的标准化模型，用于研究免疫细胞对乙醇的体内和体外反应。
