Takeuchi Y, Yoshie H, Hara K
Department of Periodontology, Niigata University School of Dentistry, Japan.
J Periodontal Res. 1991 Nov;26(6):502-10. doi: 10.1111/j.1600-0765.1991.tb01802.x.
Expression of interleukin-2 receptor (IL2R) and HLA-DR on lymphocytes of gingival crevicular fluid (GCF) was examined by two-color flow cytometric analysis. GCF from 15 patients with periodontitis was collected by crevicular washing. Mononuclear cells were isolated by Ficoll-paque gradient centrifugation from inflamed gingival tissue (GT) and peripheral blood (PB) sampled from each of the 15 patients. Lymphocyte subsets were detected by using monoclonal antibodies (mAb) of Leu 12 (CD19), Leu 4 (CD3), Leu 3a (CD4) and Leu 2a (CD8) directed to B cells, T cells, helper/inducer T cells (Th) and suppressor/cytotoxic T cells (Ts), respectively. Anti-IL2R (CD25) and anti-HLA-DR were used as lymphocyte activation markers. IL2R- or HLA-DR-positive fractions in Th, Ts and B cells were calculated. Percentage of IL2R-positive fraction in Th (IL2R+ Th) of GCF (34.0%) was significantly higher than those of GT (18.4%) and PB (13.7%). IL2R-positive fraction in B cells (IL2R+ B) of GCF was the highest among the three groups (23.9% in GCF, 12.5% in GT, 6.3% in PB). Ts did not express IL2R regardless of the origin of the samples. Compared with PB and GT, GCF showed significantly higher HLA-DR expression on Th and Ts in GCF (PB: 8.7% and 27.1%; GT: 27.9% and 50.3%; GCF: 44.7% and 65.3%). These results suggest that lymphocytes in GCF were highly activated and are related to the local host immune response in periodontitis.
采用双色流式细胞术分析检测龈沟液（GCF）中淋巴细胞白细胞介素-2受体（IL2R）和HLA-DR的表达。通过龈沟冲洗收集15例牙周炎患者的GCF。通过Ficoll-聚蔗糖密度梯度离心法从15例患者各自的炎症牙龈组织（GT）和外周血（PB）中分离单核细胞。分别使用针对B细胞、T细胞、辅助/诱导性T细胞（Th）和抑制/细胞毒性T细胞（Ts）的Leu 12（CD19）、Leu 4（CD3）、Leu 3a（CD4）和Leu 2a（CD8）单克隆抗体（mAb）检测淋巴细胞亚群。使用抗IL2R（CD25）和抗HLA-DR作为淋巴细胞活化标志物。计算Th、Ts和B细胞中IL2R或HLA-DR阳性分数。GCF中Th的IL2R阳性分数（IL2R + Th）（34.0%）显著高于GT（18.4%）和PB（13.7%）。GCF中B细胞的IL2R阳性分数（IL2R + B）在三组中最高（GCF中为23.9%，GT中为12.5%，PB中为6.3%）。无论样本来源如何，Ts均不表达IL2R。与PB和GT相比，GCF中Th和Ts上的HLA-DR表达显著更高（PB：8.7%和27.1%；GT：27.9%和50.3%；GCF：44.7%和65.3%）。这些结果表明，GCF中的淋巴细胞高度活化，与牙周炎中局部宿主免疫反应相关。
