Rondeau E, Cerrina J, Delarue F, Ladurie F L, Hervé P, Chapelier A, Dartevelle P, Sraer J D
Service de Néphrologie, Hôpital Tenon, Paris.
Presse Med. 1991 Nov 27;20(40):2007-8.
In 10 patients who received a heart lung transplant, TNF-alpha generation by cells collected during bronchioloalveolar lavages (n = 30) and by circulating mononuclear cells was measured. Basal and recombinant IL-2-stimulated productions (50 U/ml) were measured. TNF-alpha concentration was determined by an immunoradiometric assay (IRMA). Circulating mononuclear cells produced at least 4 times less TNF-alpha than BAL cells. Rejection episodes or CMV diseases were not associated with significant changes in TNF-alpha generation. Recombinant IL-2 increased this production in both cell populations but the magnitude of this effect was smaller in BAL cells, suggesting an in vivo preactivation.
在10例接受心肺移植的患者中，对支气管肺泡灌洗（n = 30）收集的细胞以及循环单核细胞产生肿瘤坏死因子-α（TNF-α）的情况进行了测定。测量了基础状态和重组白细胞介素-2（IL-2）刺激（50 U/ml）后的产生情况。TNF-α浓度通过免疫放射分析（IRMA）测定。循环单核细胞产生的TNF-α比支气管肺泡灌洗细胞至少少4倍。排斥反应或巨细胞病毒疾病与TNF-α产生的显著变化无关。重组IL-2增加了这两种细胞群体的TNF-α产生，但这种作用在支气管肺泡灌洗细胞中的幅度较小，提示存在体内预激活。
