加纳、印度尼西亚、意大利、波兰和越南散养鸡中弓形虫的血清阳性率及分离情况。
Seroprevalence and isolation of Toxoplasma gondii from free-range chickens in Ghana, Indonesia, Italy, Poland, and Vietnam.
作者信息
Dubey J P, Huong Lam Thi Thu, Lawson B W L, Subekti D T, Tassi P, Cabaj W, Sundar N, Velmurugan G V, Kwok O C H, Su C
机构信息
U.S. Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350, USA.
出版信息
J Parasitol. 2008 Feb;94(1):68-71. doi: 10.1645/GE-1362.1.
The prevalence of Toxoplasma gondii in free-ranging chickens (Gallus domesticus) is a good indicator of the prevalence of the parasite's oocysts in soil because chicken feed from the ground. The prevalence of T. gondii in free-range chickens from Ghana, Indonesia, Italy, Poland, and Vietnam was determined using the modified agglutination test (MAT). Antibodies to T. gondii were found in 41 (64%) of 64 chickens from Ghana, 24 (24.4%) of 98 chickens from Indonesia, 10 (12.5%) of 80 chickens from Italy, 6 (30%) of 20 chickens from Poland, and 81 (24.2%) of 330 chickens from Vietnam. Hearts and brains of chickens were bioassayed for T. gondii. Viable T. gondii was isolated from 2 chickens from Ghana, 1 chicken from Indonesia, 3 chickens from Italy, 2 chickens from Poland, and 1 chicken from Vietnam. Toxoplasma gondii isolates from 9 chickens were genotyped using 10 PCR-RFLP markers including SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico. A total of 7 genotypes was identified; the 3 isolates from chickens from Italy were clonal type II, and the others were nonclonal. This is the first report of genetic characterization of T. gondii isolates from animals from these countries.
散养鸡（家鸡）中弓形虫的流行情况是该寄生虫卵囊在土壤中流行情况的良好指标，因为鸡以地面上的饲料为食。采用改良凝集试验（MAT）测定了来自加纳、印度尼西亚、意大利、波兰和越南的散养鸡中弓形虫的流行情况。在来自加纳的64只鸡中有41只（64%）检测到抗弓形虫抗体，来自印度尼西亚的98只鸡中有24只（24.4%），来自意大利的80只鸡中有10只（12.5%），来自波兰的20只鸡中有6只（30%），来自越南的330只鸡中有81只（24.2%）。对鸡的心脏和大脑进行了弓形虫生物测定。从来自加纳的2只鸡、来自印度尼西亚的1只鸡、来自意大利的3只鸡、来自波兰的2只鸡和来自越南的1只鸡中分离出了活的弓形虫。使用包括SAG1、SAG2、SAG3、BTUB、GRA6、c22 - 8、c29 - 2、L358、PK1和Apico在内的10个PCR - RFLP标记对来自9只鸡的弓形虫分离株进行了基因分型。共鉴定出7种基因型；来自意大利鸡的3个分离株为克隆II型，其他为非克隆型。这是关于这些国家动物中弓形虫分离株基因特征的首次报告。
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