Mizukami Takuo, Imai Jun-ichi, Hamaguchi Isao, Kawamura Mika, Momose Haruka, Naito Seishiro, Maeyama Jun-ichi, Masumi Atsuko, Kuramitsu Madoka, Takizawa Kazuya, Nomura Nobuo, Watanabe Shinya, Yamaguchi Kazunari
Department of Safety Research on Blood and Biological Products, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan.
Vaccine. 2008 Apr 24;26(18):2270-83. doi: 10.1016/j.vaccine.2008.02.031. Epub 2008 Mar 3.
We propose that DNA microarray analysis can be used in the quality control of pandemic and endemic influenza vaccine. Based on the expression profiles of 76 genes in the rat lung one day after inoculation of influenza vaccine, we can distinguish whole-virion influenza vaccine (PDv: pandemic influenza vaccine and WPv: whole virion-particle vaccine) and sub-virion vaccine (HA vaccine) from saline. Among these 76 genes, we found genes up-regulated by influenza infection, as well as genes involved in the immune response, and interferon. Hierarchical clustering of each influenza vaccine by the expression profiles of these 76 genes matched data from current quality control tests in Japan, such as the abnormal toxicity test (ATT) and the leukopenic toxicity test (LTT). Thus, it can be concluded that DNA microarray technology is an informative, rapid and highly sensitive method with which to evaluate the quality of influenza vaccines. Using DNA microarray system, consistent with the results of the ATT and LTT, it was clarified that there was no difference in vaccine quality between PDv and WPv.
我们提出，DNA微阵列分析可用于大流行和地方性流感疫苗的质量控制。基于接种流感疫苗一天后大鼠肺中76个基因的表达谱，我们可以将全病毒流感疫苗（PDv：大流行流感疫苗和WPv：全病毒颗粒疫苗）和亚病毒疫苗（HA疫苗）与生理盐水区分开来。在这76个基因中，我们发现了受流感感染上调的基因，以及参与免疫反应和干扰素的基因。通过这76个基因的表达谱对每种流感疫苗进行层次聚类，与日本目前质量控制测试的数据相匹配，如异常毒性试验（ATT）和白细胞减少毒性试验（LTT）。因此，可以得出结论，DNA微阵列技术是一种信息丰富、快速且高度灵敏的评估流感疫苗质量的方法。使用DNA微阵列系统，与ATT和LTT的结果一致，明确了PDv和WPv之间的疫苗质量没有差异。
