Olicheva Vera, Beloborodov Vladimir, Sharifi Shamimeh, Dubrovskaya Anna, Zhevlakova Anastasiya, Selivanova Irina, Ilyasov Igor
Department of Chemistry, Nelyubin Institute of Pharmacy, Sechenov First Moscow State Medical University, Trubetskaya Street 8/2, 119048 Moscow, Russia.
Faculty of Fundamental Medicine, Lomonosov Moscow State University, 27/1 Lomonosovsky Avenue, 119991 Moscow, Russia.
Int J Mol Sci. 2025 Jun 13;26(12):5659. doi: 10.3390/ijms26125659.
The concomitant utilization of flavonoids and α-tocopherol has the potential to establish a comprehensive antioxidant system that operates in both hydrophilic and lipophilic environments. The objective of this study was to examine the antioxidant interactions between dihydroquercetin, a flavonoid that has shown promise in various studies, as well as structurally related flavonoids, and α-tocopherol in various ratios. The antioxidant capacity was assessed using two ABTS radical-cation inhibition assays: a decolorization assay and a lag-time assay. The results of this study indicated that formulations of dihydroquercetin, quercetin, rutin, or morin with α-tocopherol exhibited additive or mildly subadditive interactions, independent of their ratios. A two-phase pattern was exhibited by the lag-time data, which, in comparison with individual components, allowed us to suggest that α-tocopherol appeared to dominate the initial phase of radical scavenging, while flavonoids became active in the later phase. This finding indicates that α-tocopherol may play a role in protecting flavonoids from premature oxidation in alcoholic media. The findings could prove valuable for the rational design of antioxidant formulations in the nutraceutical, cosmeceutical, and pharmaceutical industries. Additionally, the two-stage antioxidant behavior offers prospects for the formulation of straightforward, cost-effective analytical approaches to measure components in binary antioxidant formulations.
类黄酮与α-生育酚同时使用有可能建立一个在亲水性和脂溶性环境中均起作用的综合抗氧化系统。本研究的目的是检测二氢槲皮素（一种在多项研究中显示出前景的类黄酮）以及结构相关的类黄酮与α-生育酚以不同比例混合时的抗氧化相互作用。使用两种ABTS自由基阳离子抑制试验评估抗氧化能力：脱色试验和滞后时间试验。本研究结果表明，二氢槲皮素、槲皮素、芦丁或桑色素与α-生育酚的配方表现出加和或轻度次加和相互作用，与它们的比例无关。滞后时间数据呈现出两相模式，与单个成分相比，这使我们认为α-生育酚似乎在自由基清除的初始阶段起主导作用，而类黄酮在后期变得活跃。这一发现表明α-生育酚可能在保护类黄酮在酒精介质中不被过早氧化方面发挥作用。这些发现对于营养保健品、药妆品和制药行业中抗氧化剂配方的合理设计可能具有重要价值。此外，这种两阶段抗氧化行为为制定简单、经济高效的分析方法以测量二元抗氧化剂配方中的成分提供了前景。
