Kuzon W M, Rosenblatt J D, Pynn B R, Plyley M J, McKee N H
Department of Surgery, University of Toronto, Ontario, Canada.
Microsurgery. 1991;12(5):352-60. doi: 10.1002/micr.1920120507.
In order to establish the relationship among intraoperative ischemia time, the recovery of contractile function, muscle fiber morphometry, and capillary geometry following free muscle transfer, 15 male dogs underwent unilateral, orthotopic free transfer of the left gracilis muscle with microneurovascular anastomosis. Intraoperative ischemia time varied from 0 to 4 hours. After a recovery period averaging 14 months, isometric contractile function was measured in the transfers and the contralateral, control muscles. Muscle fiber type composition, fiber size, and capillary geometry were quantified from histochemical sections. Transferred and control muscles had similar type I fiber percentages, type I fiber sizes, and capillary densities. Type II fiber sizes were reduced in transfers relative to controls. There was no significant effect of intraoperative ischemia on any morphometric variable. Although contractile function was generally reduced after free, vascularized transfer, there was only a weak correlation between the recovery of tetanic tension and type I fiber percentage (r = 0.53, P less than 0.05). It is concluded that free, vascularized muscle transfer results in minimal disturbance of muscle fiber type composition, fiber size, or capillary supply and that the diminished contractile function observed in this setting is still largely unexplained.
为了确定游离肌肉移植后术中缺血时间、收缩功能恢复、肌纤维形态计量学和毛细血管形态之间的关系，15只雄性犬接受了左股薄肌的单侧原位游离移植及显微神经血管吻合术。术中缺血时间从0到4小时不等。在平均14个月的恢复期后，测量移植肌肉和对侧对照肌肉的等长收缩功能。从组织化学切片中对肌纤维类型组成、纤维大小和毛细血管形态进行定量分析。移植肌肉和对照肌肉的I型纤维百分比、I型纤维大小和毛细血管密度相似。与对照相比，移植肌肉中II型纤维大小减小。术中缺血对任何形态计量学变量均无显著影响。尽管带血管游离移植后收缩功能普遍降低，但强直张力恢复与I型纤维百分比之间仅存在微弱相关性（r = 0.53，P < 0.05）。结论是，带血管游离肌肉移植对肌纤维类型组成、纤维大小或毛细血管供应的干扰最小，且在此情况下观察到的收缩功能减弱在很大程度上仍无法解释。
