Gridley T, Jaenisch R, Gendron-Maguire M
Department of Cell and Developmental Biology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110.
Genomics. 1991 Nov;11(3):501-7. doi: 10.1016/0888-7543(91)90056-k.
The Mov-34 mutation is a recessive embryonic lethal mutation caused by experimental introduction of a recombinant Moloney murine leukemia provirus into the mouse germline. We have cloned a full-length cDNA from the Mov-34 gene, the transcription unit disrupted by the proviral integration. This cDNA is predicted to encode a novel 321-amino acid, 36-kDa protein of unknown function. Overlapping phage lambda clones containing the entire Mov-34 gene have been isolated. The Mov-34 gene spans just over 8 kb and contains seven exons. The 5' flanking region of the Mov-34 gene contains neither "TATA" nor "CAAT" box sequences, and 5' end mapping by primer extension and ribonuclease protection reveal multiple transcription initiation sites.
Mov-34突变是一种隐性胚胎致死突变，由将重组莫洛尼氏小鼠白血病前病毒实验性导入小鼠种系引起。我们已经从Mov-34基因克隆了一个全长cDNA，该转录单位被前病毒整合破坏。预计该cDNA编码一种功能未知的新型321个氨基酸、36 kDa的蛋白质。已经分离出包含整个Mov-34基因的重叠λ噬菌体克隆。Mov-34基因跨度略超过8 kb，包含7个外显子。Mov-34基因的5'侧翼区域既不包含“TATA”盒序列也不包含“CAAT”盒序列，通过引物延伸和核糖核酸酶保护进行的5'末端定位揭示了多个转录起始位点。
