Johnson Eric F, Mukhopadhyay Biswarup
Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
Appl Environ Microbiol. 2008 Jun;74(11):3591-5. doi: 10.1128/AEM.00098-08. Epub 2008 Mar 31.
Coenzyme F(420)-dependent sulfite reductase (Fsr) of Methanocaldococcus jannaschii, a sulfite-tolerant methanogen, was expressed with activity in Methanococcus maripaludis, a sulfite-sensitive methanogen. The recombinant organism reduced sulfite to sulfide and grew with sulfite as the sole sulfur source, indicating that Fsr is a sulfite detoxification and assimilation enzyme for methanogens and that M. maripaludis synthesizes siroheme.
嗜热栖热甲烷球菌(Methanocaldococcus jannaschii)的辅酶F(420)依赖性亚硫酸盐还原酶(Fsr),一种耐亚硫酸盐的产甲烷菌,在对亚硫酸盐敏感的马氏甲烷球菌(Methanococcus maripaludis)中表达并具有活性。该重组生物体将亚硫酸盐还原为硫化物,并以亚硫酸盐作为唯一硫源生长,这表明Fsr是产甲烷菌的一种亚硫酸盐解毒和同化酶,并且马氏甲烷球菌能合成 siroheme。