Barrios Carlos A, Bañuls María José, González-Pedro Victoria, Gylfason Kristinn B, Sánchez Benito, Griol Amadeu, Maquieira A, Sohlström H, Holgado M, Casquel R
Instituto de Sistemas Optoelectrónicos y Microtecnología, Universidad Politécnica de madrid, ETSI Telecommunicación, Ciudad Universitaria s/n, Madrid, Spain.
Opt Lett. 2008 Apr 1;33(7):708-10. doi: 10.1364/ol.33.000708.
We demonstrate label-free molecule detection by using an integrated biosensor based on a Si(3)N(4)/SiO(2) slot-waveguide microring resonator. Bovine serum albumin (BSA) and anti-BSA molecular binding events on the sensor surface are monitored through the measurement of resonant wavelength shifts with varying biomolecule concentrations. The biosensor exhibited sensitivities of 1.8 and 3.2 nm/(ng/mm(2)) for the detection of anti-BSA and BSA, respectively. The estimated detection limits are 28 and 16 pg/mm(2) for anti-BSA and BSA, respectively, limited by wavelength resolution.
我们展示了通过使用基于Si(3)N(4)/SiO(2)狭缝波导微环谐振器的集成生物传感器进行无标记分子检测。通过测量随生物分子浓度变化的共振波长偏移，监测传感器表面上牛血清白蛋白（BSA）和抗BSA分子的结合事件。该生物传感器检测抗BSA和BSA的灵敏度分别为1.8和3.2 nm/(ng/mm(2))。受波长分辨率限制，抗BSA和BSA的估计检测限分别为28和16 pg/mm(2)。
