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缺氧对培养的3T6成纤维细胞中胶原蛋白合成的影响及其与抗坏血酸作用方式的关系。

The effect of hypoxia on collagen synthesis in cultured 3T6 fibroblasts and its relationship to the mode of action of ascorbate.

作者信息

Levene C I, Bates C J

出版信息

Biochim Biophys Acta. 1976 Sep 24;444(2):446-52. doi: 10.1016/0304-4165(76)90388-3.

Abstract

When exposed to low oxygen tension, in the absence of added ascorbic acid 3T6 mouse fibroblast cultures in late log phase respond by increased lactate production and increased hydroxylation of proline in nascent collagen, which is paralleled by an increase in prolyl hydroxylase activity. After 6 h recovery from the anoxic stimulus, however, cultures still yield more prolyl hydroxylase than controls, but the effect on hydroxylation of nascent collagen has disappeared. These observations help to dissect the dual role of ascorbate which can stimulate hydroxylation both by increasing the amount of active enzyme and by a cofactor-like role; in addition, these observations may be relevant to wound healing.

摘要

当暴露于低氧张力环境中时,在未添加抗坏血酸的情况下,处于对数生长期后期的3T6小鼠成纤维细胞培养物会通过增加乳酸生成以及新生胶原蛋白中脯氨酸羟化作用增强来做出反应,这与脯氨酰羟化酶活性增加同时发生。然而,在从缺氧刺激中恢复6小时后,培养物产生的脯氨酰羟化酶仍比对照多,但对新生胶原蛋白羟化作用的影响已消失。这些观察结果有助于剖析抗坏血酸盐的双重作用,它既能通过增加活性酶的量又能通过类似辅因子的作用来刺激羟化作用;此外,这些观察结果可能与伤口愈合有关。

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