Shariatmadar Sherry, Sharma Siddharth, Cabana Raquel, Powell Scott, Ruiz Phillip, Krishan Awtar
Department of Pathology, Jackson Memorial Medical Center and Sylvester Cancer Center, University of Miami Miller School of Medicine, Miami 33101, Florida, USA.
Cytometry B Clin Cytom. 2008 May;74(3):182-8. doi: 10.1002/cyto.b.20399.
The authors have used a flow analyzer to measure electronic cellular volume of peripheral blood hematopoietic stem/progenitor cells obtained by granulocyte-colony stimulating factor (G-CSF) mobilization and apheresis (HPC-A) of patients with hematological malignancies.
Fifty three apheresis samples stained with CD45-fluorescein isothiocyanate (FITC) and CD34-R-phycoerythrin (PE)-labeled antibodies after erythrocyte lysis with BD FACS Lysing Solution were analyzed for electronic cell volume and two-color FITC and PE fluorescence.
Lymphocytes, monocytes and granulocytes in the HPC-A samples had a mean electronic volume of 414, 797, and 670 microm(3), respectively corresponding to cell diameter of 9.25, 11.5, and 10.85 microm. In 53 HPC-A samples analyzed, the mean electronic volume of the CD34 positive mononuclear cells was 407 microm(3) while the CD45 positive cells had mean volume of 453 microm(3).
CD34 positive stem/progenitor cells have a smaller volume and diameter than CD45 positive mononuclear cells in HPC-A samples. In the present study the CD34 stem/progenitor cells had a considerably larger diameter than that of stem cells previously reported in the literature. With the availability of electronic cell volume as a parameter in flow cytometric analysis, further studies can be carried out to correlate stem cell volume with specific phenotypic marker expression.
作者使用流式细胞分析仪测量了血液系统恶性肿瘤患者经粒细胞集落刺激因子（G-CSF）动员和单采术（HPC-A）获取的外周血造血干/祖细胞的电子细胞体积。
用BD FACS溶血溶液裂解红细胞后，对53份单采样本进行染色，使用抗CD45-异硫氰酸荧光素（FITC）和抗CD34-藻红蛋白（PE）标记抗体，分析电子细胞体积以及FITC和PE双色荧光。
HPC-A样本中的淋巴细胞、单核细胞和粒细胞的平均电子体积分别为414、797和670立方微米，分别对应细胞直径为9.25、11.5和10.85微米。在分析的53份HPC-A样本中，CD34阳性单核细胞的平均电子体积为407立方微米，而CD45阳性细胞的平均体积为453立方微米。
在HPC-A样本中，CD34阳性干/祖细胞的体积和直径小于CD45阳性单核细胞。在本研究中，CD34干/祖细胞的直径比文献中先前报道的干细胞直径大得多。随着电子细胞体积作为流式细胞术分析中的一个参数可用，可以进行进一步研究以将干细胞体积与特定表型标志物表达相关联。
