藻红蛋白或异硫氰酸荧光素偶联的8G12在CD34+细胞评估中的差异。
Differences in phycoerythrin- or fluorescein-isothiocyanate conjugated 8G12 on CD34+ cell evaluation.
作者信息
Ortuño F, Ferrer F, Lozano M L, Heras I, Moraleda J M, Vicente V
机构信息
Unit of Hematology and Hemotherapy, School of Medicine, Hospital General Universitario, Murcia, Spain.
出版信息
Haematologica. 1997 May-Jun;82(3):334-5.
To evaluate the equivalence of 8G12 conjugated with either phycoerythrin (PE) or fluorescein isothiocyanate (FITC), duplicate fluorochrome-8G12 labelling was carried out in 229 samples. Additionally, 53 samples were simultaneously immunostained with FITC-8G12 and PE-8G12. Significantly higher values (p < 0.001) were observed in PE-CD34+ cells when compared with FITC-CD34+ cells both in duplicate and simultaneous analysis. Our data suggest that the choice of fluorochrome is relevant in the measurement of CD34+ cells with 8G12.
为评估与藻红蛋白（PE）或异硫氰酸荧光素（FITC）偶联的8G12的等效性，对229个样本进行了重复的荧光染料-8G12标记。此外，53个样本同时用FITC-8G12和PE-8G12进行免疫染色。在重复分析和同步分析中，与FITC-CD34+细胞相比，PE-CD34+细胞的值均显著更高（p < 0.001）。我们的数据表明，在使用8G12测量CD34+细胞时，荧光染料的选择至关重要。
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