拟南芥中假定的端粒结合蛋白AtTRB1和AtPOT1b相互作用结构域的定位

Mapping of interaction domains of putative telomere-binding proteins AtTRB1 and AtPOT1b from Arabidopsis thaliana.

作者信息

Schrumpfová Petra Procházková, Kuchar Milan, Palecek Jan, Fajkus Jirí

机构信息

Department of Functional Genomics and Proteomics, Institute of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, CZ-62500 Brno, Czech Republic.

出版信息

FEBS Lett. 2008 Apr 30;582(10):1400-6. doi: 10.1016/j.febslet.2008.03.034. Epub 2008 Apr 1.

DOI:10.1016/j.febslet.2008.03.034

Abstract

We previously searched for interactions between plant telomere-binding proteins and found that AtTRB1, from the single-myb-histone (Smh) family, interacts with the Arabidopsis POT1-like-protein, AtPOT1b, involved in telomere capping. Here we identify domains responsible for that interaction. We also map domains in AtTRB1 responsible for interactions with other Smh-family-members. Our results show that the N-terminal OB-fold-domain of AtPOT1b mediates the interaction with AtTRB1. This domain is characteristic for POT1- proteins and is involved with binding the G-rich-strand of telomeric DNA. AtPOT1b also interacts with AtTRB2 and AtTRB3. The central histone-globular-domain of AtTRB1 is involved with binding to AtTRB2 and 3, as well as to AtPOT1b. AtTRB1-heterodimers with other Smh-family-members are more stable than AtTRB1-homodimers. Our results reveal interaction networks of plant telomeres.

摘要

我们之前搜索了植物端粒结合蛋白之间的相互作用，发现来自单Myb-组蛋白（Smh）家族的AtTRB1与参与端粒封端的拟南芥POT1样蛋白AtPOT1b相互作用。在此，我们确定了负责这种相互作用的结构域。我们还绘制了AtTRB1中与其他Smh家族成员相互作用的结构域图谱。我们的结果表明，AtPOT1b的N端OB折叠结构域介导了与AtTRB1的相互作用。该结构域是POT1蛋白的特征性结构域，参与结合端粒DNA的富含G的链。AtPOT1b还与AtTRB2和AtTRB3相互作用。AtTRB1的中央组蛋白球状结构域参与与AtTRB2和3以及AtPOT1b的结合。AtTRB1与其他Smh家族成员的异二聚体比AtTRB1同二聚体更稳定。我们的结果揭示了植物端粒的相互作用网络。