Sun Yu, Zhang Huimin, Zou Yirong, Guo Xueqin, Feng Yuping, Liu Di, Lu Hai
State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, Beijing, 100083, China.
The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
BMC Plant Biol. 2025 Aug 1;25(1):1004. doi: 10.1186/s12870-025-06668-7.
Telomere-binding proteins play a crucial role in maintaining the stability of telomere structures. In this study, we found that GbTRB1, a double-stranded telomere-binding protein, can bind telomeric DNA., plays a pivotal role in telomere complex stability in . The Myb domain in GbTRB1 is a key domain for binding to the telomeric dsDNA sequences, while the H1/H5 histone domain is a key domain for binding to the telomeric ssDNA sequences. We the first time reported that GbTRB1 can bind telomeric DNA. GbTRB1 interacts with GbTERT, the telomerase subunit, in vivo and in vitro. The telomere length was increased in overexpressed transgenic , suggesting GbTRB1 plays an important role in maintaining telomere stability and telomere length. Our results showed GbTRB1 can bind telomeric DNA. and interact with telomerase. This unusual dual role of GbTRB1 may partly explain the longevity mechanisms observed in .
The online version contains supplementary material available at 10.1186/s12870-025-06668-7.
端粒结合蛋白在维持端粒结构的稳定性中起关键作用。在本研究中，我们发现GbTRB1，一种双链端粒结合蛋白，能够结合端粒DNA。在……中，GbTRB1在端粒复合体稳定性方面起关键作用。GbTRB1中的Myb结构域是与端粒双链DNA序列结合的关键结构域，而H1/H5组蛋白结构域是与端粒单链DNA序列结合的关键结构域。我们首次报道GbTRB1能够结合端粒DNA。GbTRB1在体内和体外均与端粒酶亚基GbTERT相互作用。在过表达的转基因……中端粒长度增加，这表明GbTRB1在维持端粒稳定性和端粒长度方面起重要作用。我们的结果表明GbTRB1能够结合端粒DNA并与端粒酶相互作用。GbTRB1这种不同寻常的双重作用可能部分解释了在……中观察到的长寿机制。
在线版本包含可在10.1186/s12870 - 025 - 06668 - 7获取的补充材料。
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