Zribi M, Ammari L, Masmoudi A, Tiouiri H, Fendri C
Laboratoire de microbiologie, hôpital la Rabota, rue Jabbari, 1007 Tunis, Tunisie.
Pathol Biol (Paris). 2009 Jul;57(5):349-52. doi: 10.1016/j.patbio.2008.02.003. Epub 2008 Apr 2.
The aim of this study was to evaluate the clinical, laboratory findings and therapeutic features of patients with brucellosis. The diagnosis was made by clinical findings, automated blood culture, serology (Rose Bengal plate agglutination test, standard tube agglutination (Wright) and immunofluorerescence). The susceptibility of 13 strains was tested in vitro. The base sequence was determined for four strains. Forty-five cases were collected (31 acute and 14 sub-acute). Contamination was digestive in 62%. Symptoms of patients were fever (93%), sweating (82%), arthralgia (78%) and splenomegaly (51%). Elevated erythrocyte sedimentation rate was determined in 80%, leukopenia in 49% and anaemia in 37% of cases. Blood cultures were positives in 39% of cases. The four sequenced strains were identified as Brucella melitensis biovar abortus. Six strains were resistant to sufomethoxazol-trimetoprim (54%). In 93% of cases, the treatment was associated rifampicin and doxycyclin. One patient died. No relapse was reported.
本研究的目的是评估布鲁氏菌病患者的临床、实验室检查结果及治疗特点。诊断依据临床症状、自动血培养、血清学检查（玫瑰红平板凝集试验、标准试管凝集试验（Wright试验）及免疫荧光法）。对13株菌株进行了体外药敏试验。测定了4株菌株的碱基序列。共收集45例病例（31例急性和14例亚急性）。62%的病例污染为消化系统污染。患者的症状有发热（93%）、出汗（82%）、关节痛（78%）和脾肿大（51%）。80%的病例红细胞沉降率升高，49%的病例白细胞减少，37%的病例贫血。39%的病例血培养呈阳性。4株测序菌株被鉴定为羊种布鲁氏菌生物变种流产布鲁氏菌。6株菌株对复方新诺明耐药（54%）。93%的病例治疗采用利福平和强力霉素联合。1例患者死亡。未报告复发情况。
