高效液相色谱-二极管阵列检测-电喷雾电离串联质谱法鉴定中药“芪参益气”滴丸中的主要成分
Identification of major constituents in the traditional Chinese medicine "QI-SHEN-YI-QI" dropping pill by high-performance liquid chromatography coupled with diode array detection-electrospray ionization tandem mass spectrometry.
作者信息
Yunfei Li, Haibin Qu, Yiyu Cheng
机构信息
Department of Chinese Medicine Science & Engineering, Zhejiang University, Hangzhou, PR China.
出版信息
J Pharm Biomed Anal. 2008 Jun 9;47(2):407-12. doi: 10.1016/j.jpba.2007.12.037. Epub 2007 Dec 31.
An HPLC-DAD-ESI-MS(n) method was developed for simultaneous analysis of major chemical constituents in "QI-SHEN-YI-QI" dropping pill, a traditional Chinese medicine (TCM) widely used for treating cardiovascular diseases. The chromatographic separation was performed on an intertsil ODS-3 C(18) column (4.6 mm x 250 mm, 5 microm), whilst water with 0.05% acetic acid and acetonitrile were used as mobile phase. On the basis of the characteristic UV absorption profile, the information of molecular weight, and structure provided by ESI-MS(n), 31 constituents derived from Astragalus membranaceus, Radix Salviae Miltiorrhizae, and Panax notoginseng, were detected and 20 of them were identified in this study. The proposed method contributes to the quality control of "QI-SHEN-YI-QI" dropping pill.
建立了一种高效液相色谱-二极管阵列检测器-电喷雾离子化质谱联用（HPLC-DAD-ESI-MS(n)）方法，用于同时分析“芪参益气”滴丸中的主要化学成分。“芪参益气”滴丸是一种广泛用于治疗心血管疾病的传统中药。色谱分离在intersil ODS-3 C(18)柱（4.6 mm×250 mm，5μm）上进行，流动相为含0.05%乙酸的水和乙腈。根据特征紫外吸收图谱、电喷雾离子化质谱提供的分子量和结构信息，在本研究中检测到了31种来源于黄芪、丹参和三七的成分，其中20种被鉴定出来。该方法有助于“芪参益气”滴丸的质量控制。
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