He Qing, Hu Xing-Jiang, Cheng Yi-Yu
Department of Chinese Medicine Science & Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China.
J Pharm Biomed Anal. 2006 May 3;41(2):485-92. doi: 10.1016/j.jpba.2006.01.010. Epub 2006 Feb 28.
An HPLC-DAD-ESI-MSn method was developed for simultaneous analysis of the chemical constituents in 'SHUANGDAN' granules, a newly developed drug widely used for treating cardiovascular disease. The chromatographic separation were performed on a Zorbax Stable Bond C18 column (4.6 mmx250 mm, 5 microm) with water with 0.5% acetic acid (A) and acetonitrile (B) as mobile phase. According to the characteristic UV absorption profile, the information of molecular weight and structure provided by ESI-MSn, 29 constituents which attributed to Radix Salviae Miltiorrhizae and Cortex Moutan, respectively, were detected and 28 constituents including 14 phenolic acids, 6 diterpenoid quinones, 6 monoterpenoids and 2 other components were identified, while some isomers were distinguished based on the MSn spectra. This method was rapid and reliable for identification of constituents in complex chemical system, 'SHUANGDAN' granules, and the newly fragmentation patterns proposed could be extended to the compounds elucidation with similar framework.
建立了一种HPLC-DAD-ESI-MSn方法，用于同时分析“双丹”颗粒中的化学成分。“双丹”颗粒是一种新开发的、广泛用于治疗心血管疾病的药物。色谱分离在Zorbax Stable Bond C18柱（4.6 mm×250 mm，5μm）上进行，以含0.5%乙酸的水（A）和乙腈（B）作为流动相。根据特征紫外吸收图谱、ESI-MSn提供的分子量和结构信息，分别检测到29种归属于丹参和牡丹皮的成分，鉴定出28种成分，包括14种酚酸、6种二萜醌、6种单萜和2种其他成分，同时根据MSn光谱区分了一些异构体。该方法对于鉴定复杂化学体系“双丹”颗粒中的成分快速且可靠，所提出的新裂解模式可扩展至对具有相似骨架的化合物的阐明。
