Mannerås Louise, Jonsdottir Ingibjörg H, Holmäng Agneta, Lönn Malin, Stener-Victorin Elisabet
Institute of Neuroscience and Physiology, Department of Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Endocrinology. 2008 Jul;149(7):3559-68. doi: 10.1210/en.2008-0053. Epub 2008 Apr 3.
Polycystic ovary syndrome (PCOS) is a complex endocrine and metabolic disorder associated with ovulatory dysfunction, hyperandrogenism, abdominal obesity, and insulin resistance. Pharmacotherapy is often unsatisfactory. This study evaluates the effects of low-frequency electro-acupuncture (EA) and physical exercise on metabolic disturbances and adipose tissue mRNA expression of selected genes in a rat PCOS model characterized by insulin resistance and adiposity. Dihydrotestosterone (inducing PCOS) or vehicle (control) was administrated continuously, beginning before puberty. At age 10 wk, PCOS rats were randomly divided into three groups; PCOS, PCOS EA, and PCOS exercise. PCOS EA rats received 2-Hz EA (evoking muscle twitches) three times/wk during 4-5 wk. PCOS exercise rats had free access to a running wheel for 4-5 wk. EA and exercise improved insulin sensitivity, measured by clamp, in PCOS rats. Exercise also reduced adiposity, visceral adipocyte size, and plasma leptin. EA increased plasma IGF-I. Real-time RT-PCR revealed increased expression of leptin and IL-6 and decreased expression of uncoupling protein 2 in visceral adipose tissue of PCOS rats compared with controls. EA restored the expression of leptin and uncoupling protein 2, whereas exercise normalized adipose tissue leptin and IL-6 expression in PCOS rats. Thus, EA and exercise ameliorate insulin resistance in rats with PCOS. This effect may involve regulation of adipose tissue metabolism and production because EA and exercise each partly restore divergent adipose tissue gene expression associated with insulin resistance, obesity, and inflammation. In contrast to exercise, EA improves insulin sensitivity and modulates adipose tissue gene expression without influencing adipose tissue mass and cellularity.
多囊卵巢综合征（PCOS）是一种复杂的内分泌和代谢紊乱疾病，与排卵功能障碍、高雄激素血症、腹部肥胖和胰岛素抵抗有关。药物治疗往往不尽人意。本研究评估了低频电针（EA）和体育锻炼对以胰岛素抵抗和肥胖为特征的大鼠PCOS模型中代谢紊乱和选定基因脂肪组织mRNA表达的影响。从青春期前开始持续给予二氢睾酮（诱导PCOS）或赋形剂（对照）。10周龄时，将PCOS大鼠随机分为三组：PCOS组、PCOS EA组和PCOS运动组。PCOS EA组大鼠在4 - 5周内每周接受3次2Hz的EA（引起肌肉抽搐）。PCOS运动组大鼠在4 - 5周内可自由使用跑步轮。通过钳夹测量发现，EA和运动改善了PCOS大鼠的胰岛素敏感性。运动还降低了肥胖程度、内脏脂肪细胞大小和血浆瘦素水平。EA增加了血浆IGF - I。实时RT - PCR显示，与对照组相比，PCOS大鼠内脏脂肪组织中瘦素和IL - 6的表达增加，解偶联蛋白2的表达降低。EA恢复了瘦素和解偶联蛋白2的表达，而运动使PCOS大鼠脂肪组织中瘦素和IL - 6的表达正常化。因此，EA和运动改善了PCOS大鼠的胰岛素抵抗。这种作用可能涉及脂肪组织代谢和生成的调节，因为EA和运动各自部分恢复了与胰岛素抵抗、肥胖和炎症相关的不同脂肪组织基因表达。与运动不同，EA在不影响脂肪组织质量和细胞构成的情况下改善胰岛素敏感性并调节脂肪组织基因表达。
