Gauthier-Rahman S, El Rouby S, Liacopoulos-Briot M, Stiffel C, Decreusefond C, Liacopoulos P
Cell Immunol. 1983 Apr 15;77(2):249-65. doi: 10.1016/0008-8749(83)90026-6.
Delayed-type hypersensitivity (DTH) and cell migration inhibition (MI) were studied in two lines of mice genetically selected for the high (Hi/PHA) or low (Lo/PHA) in vitro response of their lymphoid cells to phytochemagglutinin (PHA). A rapid photoelectric procedure for reading cell migrations enabled the study of MI over a wide range (10 log) of antigen concentrations in vitro. Hi/PHA mice required immunization with a 10 times higher dose of ovalbumin (OVA) in Freund's complete adjuvant (FCA) than Lo/PHA mice for a comparable response in DTH (footpad swelling) and MI of their induced peritoneal exudate cells (PEC). Lo/PHA spleen showed marked bizonal MI on Day 5 after immunization with low doses (0.1 and 0.5 micrograms) of OVA in FCA, one peak being obtained in presence of in vitro concentrations of 10(-3) or 10(-2) micrograms/ml OVA and another peak at 1 or 10 micrograms/ml, whereas Hi/PHA spleen showed stimulation of migration. In contrast, MI in Lo/PHA spleen failed to persist beyond Day 19, whereas it appeared progressively in Hi/PHA spleen, being maximal by Day 27. Low-zone inhibition in Hi/PHA spleen and PEC was lacking or poor even after immunization with higher doses of OVA in FCA. The implications of these findings are discussed.
在两组经基因选择的小鼠中研究了迟发型超敏反应（DTH）和细胞迁移抑制（MI），这两组小鼠的淋巴细胞对植物血凝素（PHA）的体外反应分别为高反应性（Hi/PHA）或低反应性（Lo/PHA）。一种用于读取细胞迁移的快速光电程序使得能够在体外宽范围（10个对数）的抗原浓度下研究MI。对于DTH（足垫肿胀）及其诱导的腹腔渗出细胞（PEC）的MI，Hi/PHA小鼠在弗氏完全佐剂（FCA）中需要用比Lo/PHA小鼠高10倍剂量的卵清蛋白（OVA）进行免疫才能获得可比的反应。在用FCA中低剂量（0.1和0.5微克）的OVA免疫后第5天，Lo/PHA脾脏显示出明显的双区MI，在体外OVA浓度为10^(-3)或10^(-2)微克/毫升时获得一个峰值，在1或10微克/毫升时获得另一个峰值，而Hi/PHA脾脏显示出迁移刺激。相反，Lo/PHA脾脏中的MI在第19天后未能持续，而在Hi/PHA脾脏中逐渐出现，在第27天时达到最大。即使在用FCA中更高剂量的OVA免疫后，Hi/PHA脾脏和PEC中的低区抑制也缺乏或较弱。讨论了这些发现的意义。
