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通过氢氘交换和质谱监测大分子复合物的折叠和组装。

Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry.

机构信息

Institute of Biotechnology, University of Helsinki, Finland.

出版信息

Microb Cell Fact. 2008 Apr 4;7:12. doi: 10.1186/1475-2859-7-12.

DOI:10.1186/1475-2859-7-12
PMID:18394161
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2365927/
Abstract

Recent advances in protein mass spectrometry (MS) have enabled determinations of hydrogen deuterium exchange (HDX) in large macromolecular complexes. HDX-MS became a valuable tool to follow protein folding, assembly and aggregation. The methodology has a wide range of applications in biotechnology ranging from quality control for over-expressed proteins and their complexes to screening of potential ligands and inhibitors. This review provides an introduction to protein folding and assembly followed by the principles of HDX and MS detection, and concludes with selected examples of applications that might be of interest to the biotechnology community.

摘要

近年来，蛋白质质谱（MS）的进展使得在大型大分子复合物中进行氢氘交换（HDX）的测定成为可能。HDX-MS 已成为研究蛋白质折叠、组装和聚集的有用工具。该方法在生物技术领域有广泛的应用，从过表达蛋白质及其复合物的质量控制到潜在配体和抑制剂的筛选。本文综述了蛋白质折叠和组装的原理，接着介绍了 HDX 和 MS 检测的原理，最后列举了一些可能引起生物技术界兴趣的应用实例。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a54/2365927/a80b741a539a/1475-2859-7-12-9.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a54/2365927/a80b741a539a/1475-2859-7-12-9.jpg

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Probing the cross-beta core structure of amyloid fibrils by hydrogen-deuterium exchange deep ultraviolet resonance Raman spectroscopy.
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