Zhou Dong-Hui, Ma Zhi-Min, Chen Yu
Department of Surgical Oncology, First Hospital, Medical College, Zhejiang University, Hangzhou 310003, China.
Zhonghua Zhong Liu Za Zhi. 2007 Nov;29(11):833-7.
To evaluate the correlation of CD44v6 and sCD44v6 expression with the biological activity of gastric carcinoma.
Mucosa samples from 103 gastric carcinoma patients and 10 healthy persons (control) were examined using immunohistochemical SP method; sCD44v6 level in peripheral blood samples was detected with ELISA in 86 gastric cancer patients, 30 gastric ulcer patients and 30 healthy controls. The follow-up period was 3-91 months for 88 patients in CD44v6 group and 1-91 months for 55 patients in sCD44v6 group.
(1) Positive expression of CD44v6 was found in 60.2% of gastric carcinoma, where as 0% in the normal gastric mucosa. CD44v6 protein expression was positively correlated with TNM stage, perigastric lymph node metastasis, presence of cancerous embolic angiopathy and Borrmann classification (P <0.05), but was not correlated with invasion depth, differentiation, metastasis and survival (P > 0.05); 77.8% of the patient with liver metastasis had a strong CD44v6 protein expression. (2) sCD44v6 level in the peripheral blood of gastric carcinoma patient was significantly higher than that in gastric ulcer patient or healthy control; Compared with palliative gastrectomy, sCD44v6 level significantly went down after radical operation. No relationship between sCD44v6 level and the pathological features was found, which included invasion depth, perigastric lymph node metastasis, presence of cancerous embolic angiopathy, differentiation and Borrmann classification; The survival in the high sCD44v6 level group was longer than that in low sCD44v6 level group ( P = 0.0281), but no significant difference was observed by Cox Regression analysis (P = 0.415). (3) No apparent correlation was observed between CD44v6 expression in gastric cancer and sCD44v6 concentration in peripheral blood (P>0.05).
Detection of CD44v6 expression in the gastric cancer may be helpful in evaluating the biological features and the survival in gastric carcinoma. The level of sCD44v6 in the blood may be also helpful in differential diagnosis, evaluation of surgical treatment and biological activity for gastric cancer. No apparent correlation is observed between CD44v6 expression in the cancer and that of sCD44v6 in the blood.
评估CD44v6和可溶性CD44v6（sCD44v6）表达与胃癌生物学活性的相关性。
采用免疫组织化学SP法检测103例胃癌患者和10例健康人（对照组）的黏膜样本；采用酶联免疫吸附测定法（ELISA）检测86例胃癌患者、30例胃溃疡患者和30例健康对照者外周血样本中的sCD44v6水平。CD44v6组88例患者的随访时间为3 - 91个月，sCD44v6组55例患者的随访时间为1 - 91个月。
（1）60.2%的胃癌组织中CD44v6呈阳性表达，而正常胃黏膜中CD44v6阳性表达率为0%。CD44v6蛋白表达与TNM分期、胃周淋巴结转移、癌栓血管病变及Borrmann分型呈正相关（P <0.05），但与浸润深度、分化程度、转移及生存情况无关（P >0.05）；77.8%的肝转移患者CD44v6蛋白表达强阳性。（2）胃癌患者外周血中sCD44v6水平显著高于胃溃疡患者及健康对照者；与姑息性胃切除术后相比，根治性手术后sCD44v6水平显著下降。未发现sCD44v6水平与浸润深度、胃周淋巴结转移、癌栓血管病变、分化程度及Borrmann分型等病理特征之间存在相关性；sCD44v6高水平组患者的生存期长于低水平组（P = 0.0281），但经Cox回归分析差异无统计学意义（P = 0.415）。（3）胃癌组织中CD44v6表达与外周血中sCD44v6浓度无明显相关性（P>0.05）。
检测胃癌组织中CD44v6表达可能有助于评估胃癌的生物学特性及患者生存期。血液中sCD44v6水平也可能有助于胃癌的鉴别诊断、手术治疗评估及生物学活性判断。胃癌组织中CD44v6表达与血液中sCD44v6表达无明显相关性。
