Saito H, Tsujitani S, Katano K, Ikeguchi M, Maeta M, Kaibara N
First Department of Surgery, Tottori University School of Medicine Yonago, Japan.
Cancer. 1998 Sep 15;83(6):1094-101.
The expression of variant isoforms of CD44 is correlated with the ability of tumor cells to metastasize in some clinical carcinomas. In this study, the serum concentration of soluble splice isoforms of CD44 that shared exon variant 6 (sCD44v6) were measured and the histologic expression of CD44v6 in tumors from patients with gastric carcinoma examined.
Serum samples were obtained from 102 patients with primary gastric carcinoma before surgery and serum levels of sCD44v6 were determined with an enzyme-linked immunoadsorbent assay. The expression of CD44v6 in tumors was examined by immunohistochemical staining.
Both the serum concentration of sCD44v6 and its expression in tumors were associated significantly with the depth of invasion of the tumor, lymph node metastasis, and clinical stage in patients with diffuse type gastric carcinoma. Among patients with gastric carcinoma, the serum level of sCD44v6 was higher in those with CD44v6 positive tumor cells than in those with CD44v6 negative tumor cells. The serum level of sCD44v6 was a prognostic indicator in patients with diffuse type gastric carcinoma, as was the histologic expression of CD44v6. However, neither CD44v6 nor sCD44v6 was a predictor of survival time in patients with intestinal type gastric carcinoma.
It appears that CD44v6 and sCD44v6 are related to the progression of diffuse type gastric carcinoma. An elevated serum level of sCD44v6 may be useful as a prognostic indicator in patients with diffuse type gastric carcinoma.
在某些临床癌症中，CD44变异体同工型的表达与肿瘤细胞的转移能力相关。在本研究中，检测了共享外显子变异体6（sCD44v6）的CD44可溶性剪接同工型的血清浓度，并检查了胃癌患者肿瘤中CD44v6的组织学表达。
在手术前从102例原发性胃癌患者中获取血清样本，采用酶联免疫吸附测定法测定血清sCD44v6水平。通过免疫组织化学染色检查肿瘤中CD44v6的表达。
sCD44v6的血清浓度及其在肿瘤中的表达均与弥漫型胃癌患者的肿瘤浸润深度、淋巴结转移及临床分期显著相关。在胃癌患者中，CD44v6阳性肿瘤细胞患者的血清sCD44v6水平高于CD44v6阴性肿瘤细胞患者。血清sCD44v6水平是弥漫型胃癌患者的预后指标，CD44v6的组织学表达也是如此。然而，CD44v6和sCD44v6均不是肠型胃癌患者生存时间的预测指标。
CD44v6和sCD44v6似乎与弥漫型胃癌的进展有关。血清sCD44v6水平升高可能作为弥漫型胃癌患者的预后指标。
