Tapia F J, Goihman-Yahr M, Cáceres-Dittmar G, Altieri E, Gross A, Isturiz G, Rosquete R, Viloria N, Avila-Millan E, Carrasquero M
Biomedical Institute of Central University of Venezuela.
Histol Histopathol. 1991 Jul;6(3):395-402.
Leukocyte subsets in bronchoalveolar lavage (BAL) fluid and peripheral blood of patients with paraccoccidioidomycosis, sarcoidosis and silicosis were characterized using monoclonal antibodies and an immunoperoxidase technique. In paraccocidioidomycosis, the number of T-helper/inducer CD4-positive lymphocytes was lower in peripheral blood than in BAL fluid. Additional analysis showed that the expression of HLA-DR was very similar in alveolar macrophages, lung and blood T-cells. In sarcoidosis and silicosis there were higher proportions of T-helper/inducer cells in peripheral blood than in BAL fluid. The alterations in the T-helper/inducer/T-suppressor/cytoxic CD4/CD8 ratio in sarcoidosis and silicosis were more appreciable in peripheral blood than in BAL fluid, contrasting with the results in paracoccidioidomycosis. The expression of HLA-DR by alveolar macrophages in sarcoidosis was the highest of all the disease studied. No statistically significant differences were observed between chronic multifocal and chronic unifocal paracoccidioidomycosis disease, stage II and stage III sarcoidosis, and chronic and accelerated silicosis. The three granulomatous diseases analyzed had a few alveolar macrophages expressing the CD4 molecule on their surface. These findings and the technique of analyzing both peripheral blood and BAL leukocyte subsets may help to understand the pathogenesis of interstitial lung diseases.
采用单克隆抗体和免疫过氧化物酶技术，对副球孢子菌病、结节病和矽肺患者支气管肺泡灌洗（BAL）液和外周血中的白细胞亚群进行了特征分析。在副球孢子菌病中，外周血中辅助性/诱导性T细胞CD4阳性淋巴细胞的数量低于BAL液中的数量。进一步分析表明，HLA - DR在肺泡巨噬细胞、肺和血液T细胞中的表达非常相似。在结节病和矽肺中，外周血中辅助性/诱导性T细胞的比例高于BAL液中的比例。结节病和矽肺中辅助性/诱导性T细胞/抑制性/细胞毒性T细胞CD4/CD8比值的改变在外周血中比在BAL液中更明显，这与副球孢子菌病的结果形成对比。在所有研究的疾病中，结节病患者肺泡巨噬细胞中HLA - DR的表达最高。慢性多灶性和慢性单灶性副球孢子菌病、结节病II期和III期以及慢性和加速性矽肺之间未观察到统计学上的显著差异。所分析的三种肉芽肿性疾病中，有少数肺泡巨噬细胞表面表达CD4分子。这些发现以及对外周血和BAL白细胞亚群进行分析的技术可能有助于理解间质性肺病的发病机制。
