通过表达木糖还原酶、NADP(+)依赖的木糖醇脱氢酶和木酮糖激酶的重组酿酒酵母从木糖生产生物乙醇。
Bioethanol production from xylose by recombinant Saccharomyces cerevisiae expressing xylose reductase, NADP(+)-dependent xylitol dehydrogenase, and xylulokinase.
作者信息
Matsushika Akinori, Watanabe Seiya, Kodaki Tsutomu, Makino Keisuke, Sawayama Shigeki
机构信息
Biomass Technology Research Center (BTRC), National Institute of Advanced Industrial Science and Technology (AIST), 2-2-2 Hirosuehiro, Kure, Hiroshima 737-0197, Japan.
出版信息
J Biosci Bioeng. 2008 Mar;105(3):296-9. doi: 10.1263/jbb.105.296.
We constructed a set of recombinant Saccharomyces cerevisiae strains with xylose-fermenting ability. A recombinant S. cerevisiae strain D-XR/ARSdR/XK, in which protein engineered NADP(+)-dependent XDH was expressed, showed 40% increased ethanol production and 23% decrease in xylitol excretion as compared with the reference strain D-XR/XDH/XK expressing the wild-type XDH.
我们构建了一组具有木糖发酵能力的重组酿酒酵母菌株。与表达野生型木糖醇脱氢酶（XDH）的对照菌株D-XR/XDH/XK相比，表达蛋白质工程化的烟酰胺腺嘌呤二核苷酸磷酸（NADP⁺）依赖性木糖醇脱氢酶的重组酿酒酵母菌株D-XR/ARSdR/XK，乙醇产量提高了40%，木糖醇排泄量减少了23%。
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