Zhou Da-chun, Ma Xiao-xu, Xie Jun-ran, Luo Feng, Jin Mei
Department of Anesthesiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Zhonghua Yi Xue Za Zhi. 2007 Dec 4;87(45):3224-7.
To explore the protective effects of hydroxyethyl starch (HES)(130/0.4) against ischemia reperfusion injury of kidney and its impact on the expression of intercellular adhesion molecule-1 (ICAM-1).
Forty SD rats underwent resection of the right kidney and clamping of the left renal artery for 60 min to establish renal ischemia reperfusion model, and then were randomly divided into 4 equal groups: Group S, undergoing infusion of normal saline 20 mg/kg 20 min before the reopening of the renal artery, Group W1, undergoing infusion of HES 5 mg/kg 20 min before the reopening of the renal artery, Group W2, undergoing infusion of HES 10 mg/kg, and Group W3, undergoing infusion of normal saline 20 mg/kg. Ten rats underwent sham operation and were used as controls (Group C). 24 h after reperfusion arterial blood samples were collected to detect 6 the levels of serum creatinine (Cr) and blood urea nitrogen (BUN), and then the rats were killed with their kidneys taken out to undergo pathological examination and calculation of Paller's score. Immunohistochemistry was used to detect the expression of intercellular adhesion molecule-1 (ICAM-1).
The levels of BUN and Cr of Group S were 152 +/- 22 and 3.17 +/- 1.00 mmol/L respectively, both significantly higher than those of Group C, and the levels of BUN and Cr of Groups W2 and W3 were 99 +/- 23 and 1.82 +/- 0.86 mmol/L and 92 +/- 28 and 1.57 +/- 0.70 mmol/L respectively, all significantly lower than those of Group S (P < 0. 05 or P < 0.01). The Paller's scores of W2 and W3 were 33.6 +/- 16.6 and 29.2 +/- 12.3 respectively,both significantly lower that of Group S (43.2 +/- 15. 8, both P < 0.05). The absorption levels of ICAM-1 of Groups W2 and W3 were 182 +/- 22 and 161 +/- 25 respectively, both significantly lower than that of Group S (212 +/- 32, P < 0.01).
Infusion of 10-20 ml/kg HES (130/0.4) significantly alleviates the ischemia-reperfusion injury, probably by reducing the ICAM-1 expression.
探讨羟乙基淀粉（HES）（130/0.4）对肾脏缺血再灌注损伤的保护作用及其对细胞间黏附分子1（ICAM-1）表达的影响。
40只SD大鼠行右肾切除并夹闭左肾动脉60分钟以建立肾缺血再灌注模型，然后随机分为4组，每组10只：S组，在肾动脉再开放前20分钟输注20mg/kg生理盐水；W1组，在肾动脉再开放前20分钟输注5mg/kg HES；W2组，输注10mg/kg HES；W3组，输注20mg/kg生理盐水。10只大鼠行假手术作为对照组（C组）。再灌注24小时后采集动脉血样本检测血清肌酐（Cr）和血尿素氮（BUN）水平，然后处死大鼠取出肾脏进行病理检查并计算Paller评分。采用免疫组织化学法检测细胞间黏附分子1（ICAM-1）的表达。
S组BUN和Cr水平分别为152±22和3.17±1.00mmol/L，均显著高于C组；W2组和W3组BUN和Cr水平分别为99±23和1.82±0.86mmol/L以及92±28和1.57±0.70mmol/L，均显著低于S组（P<0.05或P<0.01）。W2组和W3组的Paller评分分别为33.6±16.6和29.2±12.3，均显著低于S组（43.2±15.8，P均<0.05）。W2组和W3组ICAM-1的吸收水平分别为182±22和161±25，均显著低于S组（212±32，P<0.01）。
输注10-20ml/kg HES（130/0.4）可显著减轻缺血再灌注损伤，可能是通过降低ICAM-1表达实现的。
