[山东省屠宰场猪肝脏中戊型肝炎病毒的分离]
[Isolation of hepatitis E virus from liver of swine at abattoirs in Shandong province].
作者信息
Wang Na, Lu Yi-han, Zheng Ying-jie, Jiang Qing-wu
机构信息
Department of Epidemiology, School of Public Health, Key Laboratory on Public Health Safety, Ministry of Education, Fudan University, Shanghai, China.
出版信息
Zhonghua Liu Xing Bing Xue Za Zhi. 2007 Oct;28(10):1013-5.
OBJECTIVE
To determine the prevalence and genotype of hepatitis E virus (HEV) in swine liver before on the market and to analyze the phylogenetic relationship between the isolates from swine and human.
METHODS
35 swine liver specimens were collected from two slaughtering houses in the countryside of Shandong province, China. Nested reverse transcription-polymerase chain reaction (nested RT-PCR) and subsequent sequencing were used to determine the nucleotide sequences. A phylogenetic tree was constructed with Neighbor-joining method based on the Kimura-2-parameter model.
RESULTS
- (8.57%) of the 35 swine liver specimens being tested were positive for HEV RNA. The three swine HEV strains isolated in the present study from liver samples shared the highest identity to genotype-IV HEV.
CONCLUSION
Results from the study confirmed that HEV was detectable among swine before on the market and the genotype was the same as that representing human and swine isolates in China. It also suggested that much more attention should be paid to the safety on the digestion of swine liver.
目的
确定市售猪肝中戊型肝炎病毒（HEV）的流行情况和基因型，并分析猪源分离株与人类分离株之间的系统发育关系。
方法
从中国山东省农村的两家屠宰场收集35份猪肝标本。采用巢式逆转录-聚合酶链反应（巢式RT-PCR）及后续测序来确定核苷酸序列。基于Kimura-2-参数模型，采用邻接法构建系统发育树。
结果
35份检测的猪肝标本中有3份（8.57%）HEV RNA呈阳性。本研究从肝脏样本中分离出的三株猪HEV毒株与IV型HEV具有最高的同源性。
结论
该研究结果证实市售猪中可检测到HEV，且基因型与中国人类和猪的分离株相同。这也表明应更加关注猪肝食用的安全性。
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