Tsutsumi Tomoaki, Amakura Yoshiaki, Ashieda Kazunori, Okuyama Akira, Tanioka Youhei, Sakata Kazuto, Kobayashi Yasuo, Sasaki Kumiko, Maitani Tamio
National Institute of Health Sciences, Kamiyoga 1-18-1, Setagaya-ku, Tokyo 158-8501, Japan.
J Agric Food Chem. 2008 May 14;56(9):2867-74. doi: 10.1021/jf072490l. Epub 2008 Apr 10.
The efficacy of a combination of two enzyme-linked immunosorbent assay (ELISA) kits was examined for screening the toxic equivalent (TEQ) concentrations of dioxins in retail fish. The coplanar PCB-EIA system, which is a competitive immunoassay specific for polychlorinated biphenyl (PCB) 118, was tested as a screening method for mono- ortho PCBs. The Ah immunoassay (Ah-I), which is an ELISA-based aryl hydrocarbon receptor binding assay, was analyzed for its screening ability for non- ortho PCBs, polychlorinated dibenzo- p-dioxins (PCDDs), and dibenzofurans (PCDFs). Dilution and recovery tests using purified fish extracts revealed no major interference of the matrix in the PCB-EIA and suggested that the matrix effect was minimized in the Ah-I. Finally, the results for the fish samples ( n = 20) showed a strong correlation between this method and high-resolution gas chromatography coupled to high-resolution mass spectrometry for the determination of the TEQ concentrations of mono- ortho PCBs ( r = 0.99) and non- ortho PCBs and PCDD/Fs ( r = 0.97). These data indicate that our method is suitable for screening retail fish to determine the TEQ concentrations of dioxins.
研究了两种酶联免疫吸附测定（ELISA）试剂盒联合使用在筛查零售鱼类中二恶英毒性当量（TEQ）浓度方面的效果。共面多氯联苯酶免疫分析（PCB-EIA）系统是一种针对多氯联苯（PCB）118的竞争性免疫分析方法，被用作单邻位多氯联苯的筛查方法进行了测试。芳烃免疫分析（Ah-I）是一种基于ELISA的芳烃受体结合分析方法，对其在非邻位多氯联苯、多氯二苯并对二恶英（PCDDs）和二苯并呋喃（PCDFs）筛查能力方面进行了分析。使用纯化的鱼类提取物进行的稀释和回收率测试表明，基质对PCB-EIA没有重大干扰，并表明在Ah-I中基质效应已降至最低。最后，对鱼类样品（n = 20）的检测结果显示，该方法与高分辨率气相色谱-高分辨率质谱法在测定单邻位多氯联苯的TEQ浓度（r = 0.99）以及非邻位多氯联苯和PCDD/Fs的TEQ浓度（r = 0.97）方面具有很强的相关性。这些数据表明，我们的方法适用于筛查零售鱼类以确定二恶英的TEQ浓度。
