Peck B K, Vanderwolf C H
Department of Psychology, University of Western Ontario, London, Canada.
Brain Res. 1991 Dec 24;568(1-2):244-52. doi: 10.1016/0006-8993(91)91404-o.
In chronically prepared rats, electrical stimulation (100 Hz, 0.1 ms pulses) of the dorsal raphe nucleus, some sites in the median raphe nucleus, and adjoining regions of the midbrain produced locomotion accompanied by hippocampal rhythmical slow activity (RSA) and neocortical low voltage fast activity (LVFA). Both the behaviour and the cerebral waveforms persisted after injection of scopolamine HBr (5 mg/kg, s.c.). Median raphe stimulation usually produced behavioural freezing or an unnatural forced movement accompanied by RSA and LVFA. The behavioural response and the LVFA were not affected by scopolamine but scopolamine eliminated the RSA, replacing it with a low amplitude irregular (suppressed) pattern. p-Chlorophenylalanine (PCPA, 500 mg/kg/day x 3, i.p.) reduced the RSA and LVFA normally present during walking after scopolamine but did not reduce the hippocampal suppression produced by median raphe stimulation in scopolamine-treated rats. Hippocampal suppression and LVFA in response to median raphe stimulation were also present in urethane (1.0-1.5 g/kg, i.p.) anesthetized rats, whether pretreated with PCPA or not. Stimulation at most other midbrain sites produced RSA and LVFA in the urethane condition. RSA was abolished in the urethane plus scopolamine condition. The data support the view that scopolamine-resistant RSA and LVFA are dependent on serotonergic projections. The hippocampal suppression produced by median raphe stimulation may be dependent on non-serotonergic neurotransmission.
在长期制备的大鼠中，对中缝背核、中缝正中核的一些部位以及中脑相邻区域进行电刺激（100赫兹，0.1毫秒脉冲）可产生运动，并伴有海马节律性慢活动（RSA）和新皮质低电压快活动（LVFA）。注射氢溴酸东莨菪碱（5毫克/千克，皮下注射）后，行为和脑电波波形仍持续存在。中缝正中核刺激通常会产生行为静止或不自然的强迫运动，并伴有RSA和LVFA。行为反应和LVFA不受东莨菪碱影响，但东莨菪碱消除了RSA，取而代之的是低振幅不规则（抑制）模式。对氯苯丙氨酸（PCPA，500毫克/千克/天×3，腹腔注射）可降低东莨菪碱处理后行走时正常出现的RSA和LVFA，但不能降低东莨菪碱处理大鼠中缝正中核刺激所产生的海马抑制。无论是预先用PCPA处理还是未处理，在氨基甲酸乙酯（1.0 - 1.5克/千克，腹腔注射）麻醉的大鼠中，对中缝正中核刺激的海马抑制和LVFA也存在。在氨基甲酸乙酯条件下，刺激大多数其他中脑部位会产生RSA和LVFA。在氨基甲酸乙酯加东莨菪碱条件下，RSA被消除。这些数据支持这样一种观点，即对东莨菪碱耐药的RSA和LVFA依赖于5-羟色胺能投射。中缝正中核刺激所产生的海马抑制可能依赖于非5-羟色胺能神经传递。
