Witlox K J, Nguyen T N, Bruggink L D, Catton M G, Marshall J A
Victorian Infectious Diseases Reference Laboratory, 10 Wreckyn Street, North Melbourne, Victoria 3051, Australia.
J Virol Methods. 2008 Jun;150(1-2):70-2. doi: 10.1016/j.jviromet.2008.02.010. Epub 2008 Apr 8.
The aim of the study was to compare the sensitivity of a norovirus RT-PCR method using two manual RNA extraction methods (Qiagen and Roche) and two automated RNA extraction methods (Qiagen and Corbett). All four RNA extraction methods gave similar sensitivities although the automated methods, especially the Corbett, required significantly less labour than the manual methods. The automated methods also enabled RNA extraction of approximately two to three times the number of specimens in a given time period compared to manual methods.
该研究的目的是比较使用两种手动RNA提取方法（Qiagen和Roche）和两种自动RNA提取方法（Qiagen和Corbett）的诺如病毒RT-PCR方法的灵敏度。尽管自动方法，尤其是Corbett方法，所需的人力比手动方法少得多，但所有四种RNA提取方法的灵敏度相似。与手动方法相比，自动方法还能够在给定时间段内提取大约两到三倍数量的标本的RNA。
