McCarty William J, Johnson Mark
Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA.
Biorheology. 2007;44(5-6):303-17.
In this study, we measured the specific hydraulic conductivity (K) of Matrigel at 1% and 2% concentrations as a function of perfusion pressure (0 to 100 mmHg) and compared the results to predictions from two models: a fiber matrix model that predicted K of the gel based upon its composition, and a biphasic model that predicted changes in K caused by pressure induced compaction of the gels. The extent of gel compaction as a function of perfusion pressure was also assessed, allowing us to estimate the stiffness of the gels. As expected, 2% Matrigel had a lower K and a higher stiffness than did 1% Matrigel. Measured values of K of both 1% and 2% Matrigel samples showed good agreement with the predictions of the fiber matrix model. Pressure-induced changes in K were better described by the biphasic model than a model in which uniform compression of the gel was assumed. We conclude that K of multi-component gels, such as Matrigel can be well characterized by fiber matrix models, and that pressure-induced changes in K of such gels can be well characterized by biphasic models.
在本研究中，我们测量了浓度为1%和2%的基质胶的比水力传导率（K）作为灌注压力（0至100 mmHg）的函数，并将结果与两个模型的预测进行了比较：一个基于凝胶成分预测其K值的纤维基质模型，以及一个预测压力诱导凝胶压实导致K值变化的双相模型。还评估了凝胶压实程度作为灌注压力的函数，使我们能够估计凝胶的硬度。正如预期的那样，2%的基质胶比1%的基质胶具有更低的K值和更高的硬度。1%和2%基质胶样品的K值测量结果与纤维基质模型的预测结果吻合良好。双相模型比假设凝胶均匀压缩的模型能更好地描述压力诱导的K值变化。我们得出结论，多组分凝胶（如基质胶）的K值可以通过纤维基质模型很好地表征，并且此类凝胶压力诱导的K值变化可以通过双相模型很好地表征。
