Zhang C, Bills M, Quigley A, Maxwell R J, Linnane A W, Nagley P
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.
Biochem Biophys Res Commun. 1997 Jan 23;230(3):630-5. doi: 10.1006/bbrc.1996.6020.
The prevalence in tissues of mtDNA deletions was compared by PCR between humans and rats of similar "biological ages". Pairs of species-specific primers were used which spanned similar portions of the human and rat mtDNA genomes. There were much fewer PCR products amplified from rat mtDNA than from human mtDNA in each of the three tissues initially analysed: heart, liver and skeletal muscle. By contrast, many more PCR products were amplified from rat kidney than from human kidney. Therefore, while there were far more deletions in heart, liver and skeletal muscle of humans than in corresponding rat tissues, the prevalence of mtDNA deletions was markedly less in human kidney than in rat kidney. The data also indicate that human kidney contains less mtDNA deletions than heart, liver and skeletal muscle in humans; whereas in rat kidney there are more mtDNA deletions than in those three tissues of rat. It is further suggested that, when utilising rodents as experimental models for human ageing, the appropriate tissues should be considered, since not all tissues of rats accumulate mtDNA mutations in the same manner as those of humans.
通过聚合酶链反应（PCR）比较了“生物学年龄”相似的人类和大鼠组织中mtDNA缺失的发生率。使用了跨越人类和大鼠线粒体DNA（mtDNA）基因组相似部分的物种特异性引物对。在最初分析的三种组织（心脏、肝脏和骨骼肌）中，从大鼠mtDNA扩增出的PCR产物比从人类mtDNA扩增出的要少得多。相比之下，从大鼠肾脏扩增出的PCR产物比从人类肾脏扩增出的要多得多。因此，虽然人类心脏、肝脏和骨骼肌中的缺失比相应大鼠组织中的多得多，但人类肾脏中mtDNA缺失的发生率明显低于大鼠肾脏。数据还表明，人类肾脏中的mtDNA缺失比人类心脏、肝脏和骨骼肌中的少；而在大鼠肾脏中，mtDNA缺失比大鼠的这三种组织中的多。进一步表明，在将啮齿动物用作人类衰老的实验模型时，应考虑合适的组织，因为并非大鼠的所有组织都以与人类相同的方式积累mtDNA突变。
