Ruby J D, Lux R, Shi W, Charon N W, Dasanayake A
Department of Pediatric Dentistry, School of Dentistry, The University of Alabama at Birmingham, Birmingham, AL 35294, USA.
Oral Microbiol Immunol. 2008 Jun;23(3):234-8. doi: 10.1111/j.1399-302X.2007.00417.x.
Treponema denticola inhabits the oral subgingival environment and is part of a proteolytic benzoyl-dl-arginine-naphthylamide-positive 'red complex' associated with active periodontal disease. Spirochetes have a unique form of chemotactic motility that may contribute to their virulence. Chemotaxis is essential for efficient nutrient-directed translocation.
We examined the effect of glucose on T. denticola cell velocity, expression of periplasmic flagella proteins, and chemotaxis, e.g. translocation into capillary tubes.
The presence of glucose did not significantly effect T. denticola cell velocity in high viscosity conditions nor did it alter periplasmic flagella protein expression. The addition of glucose to capillary tubes resulted in greater numbers of T. denticola cells in tubes containing glucose. A non-motile mutant did not migrate into capillary tubes containing glucose.
These results are consistent with a chemotactic response to glucose that is motility dependent.
齿垢密螺旋体栖息于口腔龈下环境，是与活动性牙周病相关的蛋白水解性苯甲酰 - dl - 精氨酸 - 萘酰胺阳性“红色复合体”的一部分。螺旋体具有独特的趋化运动形式，这可能有助于其致病性。趋化作用对于有效的营养物质导向转运至关重要。
我们研究了葡萄糖对齿垢密螺旋体细胞速度、周质鞭毛蛋白表达以及趋化作用（如向毛细管内转运）的影响。
在高粘度条件下，葡萄糖的存在对齿垢密螺旋体细胞速度没有显著影响，也未改变周质鞭毛蛋白的表达。向毛细管中添加葡萄糖导致含有葡萄糖的管内齿垢密螺旋体细胞数量增加。一个非运动性突变体不会迁移到含有葡萄糖的毛细管中。
这些结果与对葡萄糖的趋化反应是运动依赖性的一致。
