Grenier Daniel
Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, 2420 de la Terrasse, Quebec City, QC, Canada G1V 0A6.
Int J Dent. 2013;2013:305476. doi: 10.1155/2013/305476. Epub 2013 Jan 13.
Porphyromonas gingivalis sheds outer membrane vesicles that contain several virulence factors, including adhesins. In this study, we investigated the ability of P. gingivalis outer membrane vesicles to mediate the coaggregation and piggybacking of Treponema denticola and Lachnoanaerobaculum saburreum. Marked coaggregation between T. denticola and L. saburreum occurred in the presence of P. gingivalis outer membrane vesicles. Sucrose was an effective chemoattractant for the motile species T. denticola. The addition of outer membrane vesicles to a mixture of T. denticola and L. saburreum significantly increased the number of nonmotile bacteria that migrated into a sucrose-filled capillary tube immersed in the bacterial mixture. Under optimal conditions, the number of nonmotile L. saburreum in the capillary tube increased approximately 5-fold, whereas no increase occurred when boiled vesicles were used. This study showed that P. gingivalis outer membrane vesicles mediate coaggregation between T. denticola and L. saburreum and that nonmotile bacteria can be translocated by piggybacking on spirochetes.
牙龈卟啉单胞菌会释放包含多种毒力因子（包括黏附素）的外膜囊泡。在本研究中，我们调查了牙龈卟啉单胞菌外膜囊泡介导具核梭杆菌和萨氏栖瘤短杆菌共聚集及搭载的能力。在牙龈卟啉单胞菌外膜囊泡存在的情况下，具核梭杆菌和萨氏栖瘤短杆菌之间发生了明显的共聚集。蔗糖是运动性菌种具核梭杆菌的一种有效化学引诱剂。将外膜囊泡添加到具核梭杆菌和萨氏栖瘤短杆菌的混合物中，显著增加了迁移到浸入细菌混合物中的充满蔗糖的毛细管中的非运动性细菌数量。在最佳条件下，毛细管中非运动性萨氏栖瘤短杆菌的数量增加了约5倍，而使用煮沸的囊泡时则没有增加。本研究表明，牙龈卟啉单胞菌外膜囊泡介导具核梭杆菌和萨氏栖瘤短杆菌之间的共聚集，并且非运动性细菌可以通过搭载螺旋体进行转移。
