Chassot Francieli, Negri Melyssa F N, Svidzinski Arthur E, Donatti Lucélia, Peralta Rosane M, Svidzinski Terezinha I E, Consolaro Marcia E L
Division of Clinical Cytology, Teaching and Research in Clinical Analysis Laboratory, State University of Maringá, Maringá, Paraná, Brazil.
Contraception. 2008 May;77(5):355-9. doi: 10.1016/j.contraception.2008.01.007. Epub 2008 Mar 19.
The in vitro adherence of Candida albicans isolated from vaginal exudates of patients with vulvovaginal candidiasis (VVC) to intrauterine contraceptive devices (IUDs) and biofilm formation capacity were evaluated.
This research was conducted with two vaginal C. albicans isolates. The adherence on IUD by both radiomarked adhesion assay and scanning electron microscopy, and the biofilm production capacity by spectrophotometric method were determined.
The yeasts adhered strongly to different parts of the IUD (covered with copper wire, without copper wire and tail), and there was no significant difference in the rates of adhesion to the different parts (p=.7771). The vaginal yeasts showed a high capacity to produce biofilm.
Two vaginal yeasts evaluated showed a high capacity to produce biofilm on IUD. It was confirmed that all parts of the IUD allow the adherence of yeasts. The adherence of C. albicans to different parts of the IUD and its formation of biofilm seems to be important attributes influencing the occurrence of VVC and recurrent VVC.
评估从外阴阴道念珠菌病（VVC）患者阴道分泌物中分离出的白色念珠菌对宫内节育器（IUD）的体外黏附情况及其生物膜形成能力。
本研究使用了两株阴道白色念珠菌分离株。通过放射性标记黏附试验和扫描电子显微镜测定其对IUD的黏附情况，并用分光光度法测定生物膜产生能力。
酵母强烈黏附于IUD的不同部位（覆盖铜线、未覆盖铜线及尾部），对不同部位的黏附率无显著差异（p = 0.7771）。阴道酵母显示出较高的生物膜产生能力。
所评估的两株阴道酵母在IUD上显示出较高的生物膜产生能力。证实IUD的所有部位均允许酵母黏附。白色念珠菌对IUD不同部位的黏附及其生物膜形成似乎是影响VVC和复发性VVC发生的重要因素。
