Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.
J Assist Reprod Genet. 2011 Jul;28(7):643-9. doi: 10.1007/s10815-011-9563-3. Epub 2011 May 4.
To investigate the effects of cryo-storage duration in liquid nitrogen on oocyte cryo-survival, fertilization and embryonic development following vitrification and warming.
Mature mouse oocytes were vitrified with McGill Cryoleaf and stored in liquid nitrogen for a period of 8-10 days, 90-92 days and 180-182 days, respectively. After warming, the survived oocytes were inseminated by intracytoplasmic sperm injection (ICSI) and cultured for 120 h. The rates of oocyte cryo-survival, cleavage and embryonic development were compared.
RESULT(S): The oocyte cryo-survival rate declined following cryo-storage duration for 180-182 days (90.4 ± 7.9%) compared to that of the other two groups (97.4 ± 3.0% and 98.0 ± 3.3%). The fertilization rate in the group of 180-182 days (66.6 ± 22.0%) was also significantly reduced (P < 0.05) compared with the groups of 8-10 days (92.2 ± 10.8%) and 90-92 days (94.7 ± 9.1%). In addition, the number of embryos developed to the blastocyst stage declined significantly (P < 0.05) following long cryo-storage duration (72.1 ± 8.2%, 25.2 ± 3.8% and 5.5 ± 13.6%, respectively).
CONCLUSION(S): The cryo-survival, fertilization rate and embryonic development of mouse oocytes are affected significantly, in an adverse manner, by the cryo-storage duration in liquid nitrogen.
研究液氮中Cryo-Storage 持续时间对玻璃化冷冻和解冻后卵母细胞冷冻存活、受精和胚胎发育的影响。
采用 McGill Cryoleaf 对成熟的小鼠卵母细胞进行玻璃化冷冻，分别在液氮中储存 8-10 天、90-92 天和 180-182 天。解冻后，通过胞浆内单精子注射（ICSI）使存活的卵母细胞受精，并培养 120 小时。比较卵母细胞的冷冻存活率、卵裂率和胚胎发育率。
与其他两组（97.4±3.0%和 98.0±3.3%）相比，Cryo-Storage 持续 180-182 天的卵母细胞冷冻存活率下降（90.4±7.9%）。180-182 天组的受精率（66.6±22.0%）也明显降低（P<0.05），而 8-10 天组和 90-92 天组的受精率分别为 92.2±10.8%和 94.7±9.1%。此外，随着 Cryo-Storage 持续时间的延长，胚胎发育到囊胚阶段的数量明显减少（P<0.05），分别为 72.1±8.2%、25.2±3.8%和 5.5±13.6%。
液氮中 Cryo-Storage 持续时间对小鼠卵母细胞的冷冻存活率、受精率和胚胎发育有显著的不利影响。
