Chunli Zheng, Jiti Zhou, Jing Wang, Jing Wang, Baocheng Qu
School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian, Liaoning Province, People's Republic of China.
J Hazard Mater. 2008 Dec 15;160(1):194-9. doi: 10.1016/j.jhazmat.2008.02.101. Epub 2008 Mar 4.
Strain Z1 was isolated from nitrobenzene-contaminated sludge. Strain Z1 was able to utilize nitrobenzene as a sole source of carbon, nitrogen, and energy under aerobic condition. Based on the morphology, physiological biochemical characteristics, and 26S rDNA D1/D2 domain sequence, strain Z1 was identified as Rhodotorula mucilaginosa. Strain Z1 mineralized up to 450mg L(-1) nitrobenzene. Kinetics of nitrobenzene degradation was described using the Andrews equation. The kinetic parameters were as follows: q(max)=1.50h(-1), K(s)=31.31mg L(-1), and K(i)=101.34mg L(-1). Strain Z1 had a high-salinity tolerance. It degraded nitrobenzene effectively in 5% NaCl (quality concentration). Even in the presence of aniline or phenol, strain Z1 degraded nitrobenzene efficiently. Strain Z1 therefore could be an excellent candidate for the bio-treatment of nitrobenzene industrial wastewaters.
菌株Z1从受硝基苯污染的污泥中分离得到。菌株Z1能够在有氧条件下将硝基苯作为唯一的碳源、氮源和能源加以利用。基于形态学、生理生化特性以及26S rDNA D1/D2结构域序列，菌株Z1被鉴定为粘红酵母。菌株Z1可将高达450mg L(-1)的硝基苯矿化。使用安德鲁斯方程描述了硝基苯的降解动力学。动力学参数如下：q(max)=1.50h(-1)，K(s)=31.31mg L(-1)，K(i)=101.34mg L(-1)。菌株Z1具有高盐耐受性。它能在5% NaCl（质量浓度）中有效降解硝基苯。即使存在苯胺或苯酚，菌株Z1也能高效降解硝基苯。因此，菌株Z1可能是硝基苯工业废水生物处理的极佳候选菌株。
