Sahu S S, Gunasekaran K, Jambulingam P, Krishnamoorthy N
Vector Control Research Centre (ICMR), Puducherry, India.
Indian J Med Res. 2008 Feb;127(2):178-82.
BACKGROUND & OBJECTIVE: Keonjhar district of Orissa State has been hyperendemic for falciparum malaria since many years with alarming deaths due to cerebral malaria. Therefore an entomological investigation to know more about the relative prevalence of Anopheles species was done.
Daytime indoor resting and outdoor resting, light trap and double bed net collections were made. Surveys were also made to collect Anopheles immature in streams and paddy fields. The Anopheles mosquitoes obtained by different catching methods were identified and the known vector species were subjected to gut and salivary gland dissection for vector incrimination. The infected specimens of An. fluviatilis and An. minimus were subjected to polymerase chain reaction assay for identification of sibling species.
Of the anophelines collected, the most abundant was An. splendidus (18.2%) and An. fluviatilis (17.7%), followed by An. maculatus (14.0%) and An. minimus (9.0%). The sporozoite rate of An. fluviatilis and An. minimus was 0.9 and 1.4 respectively. The infected specimens have been identified as sibling species S of the An. fluviatilis complex and A of the An. minimus complex.
INTERPRETATION & CONCLUSION: An. fluviatilis and An. minimus are the major two species in the transmission of malaria in Keonjhar district in Orissa.
多年来，奥里萨邦的科恩贾尔地区一直是恶性疟的高度流行区，因脑型疟导致的死亡人数惊人。因此，开展了一项昆虫学调查，以进一步了解按蚊种类的相对流行情况。
进行了白天室内栖息和室外栖息采集、灯光诱捕和双层床蚊帐采集。还开展了调查，以收集溪流和稻田中的按蚊幼虫。对通过不同捕捉方法获得的按蚊进行鉴定，并对已知的传病媒介种类进行肠道和唾液腺解剖以确定传病媒介。对感染的溪流按蚊和微小按蚊标本进行聚合酶链反应分析，以鉴定同域物种。
在采集的按蚊中，数量最多的是华丽按蚊（18.2%）和溪流按蚊（17.7%），其次是多斑按蚊（14.0%）和微小按蚊（9.0%）。溪流按蚊和微小按蚊的子孢子率分别为0.9和1.4。已将感染标本鉴定为溪流按蚊复合体的同域物种S和微小按蚊复合体的A。
溪流按蚊和微小按蚊是奥里萨邦科恩贾尔地区疟疾传播的主要两种按蚊。
