Ates Burhan, Abraham Linu, Ercal Nuran
Department of Chemistry, Missouri University of Science and Technology, Rolla, MO 65409, USA.
Free Radic Res. 2008 Apr;42(4):372-7. doi: 10.1080/10715760801998638.
The antioxidant potential of N-acetylcysteine amide (NACA), also known as AD4, was assessed by employing different in vitro assays. These included reducing power, free radical scavenging capacities, peroxidation inhibiting activity through linoleic acid emulsion system and metal chelating capacity, as compared to NAC and three widely used antioxidants, alpha-tocopherol, ascorbic acid and butylated hydroxytoluene (BHT). Of the antioxidant properties that were investigated, NACA was shown to possess higher 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging ability and reducing power than NAC, at all the concentrations, whereas the scavenging ability of H(2)O(2) differed with concentration. While NACA had greater H(2)O(2) scavenging capacity at the highest concentration, NAC was better than NACA at lower concentrations. NAC and NACA had a 60% and 55% higher ability to prevent beta-carotene bleaching, respectively, as compared to control. The chelating activity of NACA was more than 50% that of the metal chelating capacity of EDTA and four and nine times that of BHT and alpha-tocopherol, respectively. When compared to NACA and NAC; alpha-tocopherol had higher DPPH scavenging abilities and BHT and alpha-tocopherol had better beta-carotene bleaching power. These findings provide evidence that the novel antioxidant, NACA, has indeed enhanced the antioxidant properties of NAC.
通过采用不同的体外试验评估了N-乙酰半胱氨酸酰胺（NACA），也称为AD4的抗氧化潜力。与NAC以及三种广泛使用的抗氧化剂α-生育酚、抗坏血酸和丁基羟基甲苯（BHT）相比，这些试验包括还原能力、自由基清除能力、通过亚油酸乳液体系的过氧化抑制活性和金属螯合能力。在所研究的抗氧化特性中，NACA在所有浓度下均显示出比NAC更高的2,2-二苯基-1-苦基肼基水合物（DPPH）自由基清除能力和还原能力，而H₂O₂的清除能力随浓度而变化。虽然NACA在最高浓度下具有更大的H₂O₂清除能力，但在较低浓度下NAC优于NACA。与对照相比，NAC和NACA分别具有高60%和55%的防止β-胡萝卜素漂白的能力。NACA的螯合活性分别超过EDTA金属螯合能力的50%以上，以及BHT和α-生育酚的四倍和九倍。与NACA和NAC相比，α-生育酚具有更高的DPPH清除能力，BHT和α-生育酚具有更好的β-胡萝卜素漂白能力。这些发现提供了证据，表明新型抗氧化剂NACA确实增强了NAC的抗氧化特性。
