Gülçin Ilhami
Atatürk University, Faculty of Arts and Sciences, Department of Chemistry, TR-25240-Erzurum-Turkey.
Life Sci. 2006 Jan 18;78(8):803-11. doi: 10.1016/j.lfs.2005.05.103. Epub 2005 Oct 25.
L-carnitine plays an important regulatory role in the mitochondrial transport of long-chain free fatty acids. In this study, the antioxidant activity of L-carnitine was investigated as in vitro. The antioxidant properties of the L-carnitine were evaluated by using different antioxidant assays such as 1, 1-diphenyl-2-picryl-hydrazyl free radical (DPPH.) scavenging, total antioxidant activity, reducing power, superoxide anion radical scavenging, hydrogen peroxide scavenging and metal chelating activities. Total antioxidant activity was measured according to ferric thiocyanate method. alpha-tocopherol and trolox, a water-soluble analogue of tocopherol, were used as the reference antioxidant compounds. At the concentrations of 15, 30 and 45 microg/mL, l-carnitine showed 94.6%, 95.4% and 97.1% inhibition on lipid peroxidation of linoleic acid emulsion, respectively. On the other hand, 45 microg/mL of standard antioxidant such as alpha-tocopherol and trolox indicated an inhibition of 88.8% and 86.2% on peroxidation of linoleic acid emulsion, respectively. In addition, L-carnitine had an effective DPPH. scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging, total reducing power and metal chelating on ferrous ions activities. Also, those various antioxidant activities were compared to alpha-tocopherol and trolox as references antioxidants.
左旋肉碱在长链游离脂肪酸的线粒体转运中发挥着重要的调节作用。在本研究中，对左旋肉碱的抗氧化活性进行了体外研究。通过使用不同的抗氧化分析方法，如1,1-二苯基-2-苦基肼自由基（DPPH·）清除、总抗氧化活性、还原能力、超氧阴离子自由基清除、过氧化氢清除和金属螯合活性，来评估左旋肉碱的抗氧化特性。总抗氧化活性根据硫氰酸铁法测定。α-生育酚和生育酚的水溶性类似物托洛克斯用作参考抗氧化化合物。在15、30和45微克/毫升的浓度下，左旋肉碱对亚油酸乳液脂质过氧化的抑制率分别为94.6%、95.4%和97.1%。另一方面，45微克/毫升的标准抗氧化剂如α-生育酚和托洛克斯对亚油酸乳液过氧化的抑制率分别为88.8%和86.2%。此外，左旋肉碱对DPPH·清除、超氧阴离子自由基清除、过氧化氢清除、总还原能力以及对亚铁离子的金属螯合活性均有效。同时，将这些不同的抗氧化活性与作为参考抗氧化剂的α-生育酚和托洛克斯进行了比较。
