吗啡的体外抗氧化特性。

In vitro antioxidant properties of morphine.

作者信息

Gülçin Ilhami, Beydemir Sükrü, Alici H Ahmet, Elmastaş Mahfuz, Büyükokuroğlu M Emin

机构信息

Department of Chemistry, Science and Arts Faculty, Atatürk University, 25240 Erzurum, Turkey.

出版信息

Pharmacol Res. 2004 Jan;49(1):59-66. doi: 10.1016/j.phrs.2003.07.012.

DOI:10.1016/j.phrs.2003.07.012

PMID:14597153

Abstract

Morphine is implicated in diverse functions, from development to immune modulation in the central and peripheral nervous systems. It has also been used extensively in the clinical management of pain due to its potent analgesic effect. This study was designed to evaluate the in vitro antioxidant capacity of morphine using different antioxidant tests, including total antioxidant activity, reducing power, free radical scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging and metal chelating activities. Morphine exhibited strong total antioxidant activity. The concentrations of 25, 50 and 75 microgml(-1) of morphine showed 79.1, 84.3 and 92.3% inhibition on peroxidation of linoleic acid emulsion, respectively. On the other hand, at 75 microgml(-1) concentration of standard antioxidant, such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and alpha-tocopherol, exhibited 88.7, 94.5 and 70.4% inhibition on peroxidation of linoleic acid emulsion, respectively. In addition, morphine had effective reducing power, free radical scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging and metal chelating activities at the same concentrations (25, 50 and 75 microgml(-1)). These various antioxidant activities were compared to standard antioxidants such as BHA, BHT and alpha-tocopherol.

摘要

吗啡具有多种功能，从发育到中枢和外周神经系统的免疫调节。由于其强大的镇痛作用，它也被广泛用于疼痛的临床管理。本研究旨在使用不同的抗氧化测试来评估吗啡的体外抗氧化能力，包括总抗氧化活性、还原能力、自由基清除、超氧阴离子自由基清除、过氧化氢清除和金属螯合活性。吗啡表现出很强的总抗氧化活性。25、50和75微克/毫升浓度的吗啡对亚油酸乳液过氧化的抑制率分别为79.1%、84.3%和92.3%。另一方面，在75微克/毫升浓度的标准抗氧化剂如丁基羟基茴香醚（BHA）、丁基羟基甲苯（BHT）和α-生育酚对亚油酸乳液过氧化的抑制率分别为88.7%、94.5%和70.4%。此外，吗啡在相同浓度（25、50和75微克/毫升）下具有有效的还原能力、自由基清除、超氧阴离子自由基清除、过氧化氢清除和金属螯合活性。将这些各种抗氧化活性与标准抗氧化剂如BHA、BHT和α-生育酚进行了比较。