七鳃鳗视紫红质cDNA的分离与鉴定
Isolation and characterization of lamprey rhodopsin cDNA.
作者信息
Hisatomi O, Iwasa T, Tokunaga F, Yasui A
机构信息
Department of Physics, Faculty of Science, Tohoku University, Sendai, Japan.
出版信息
Biochem Biophys Res Commun. 1991 Feb 14;174(3):1125-32. doi: 10.1016/0006-291x(91)91537-m.
Genomic DNA fragments coding a visual pigment of the lamprey were amplified by polymerase chain reaction, using oligonucleotide mixtures as primers. The complete coding region of the cDNA was obtained by separate amplification of both cDNA ends. The deduced amino acid sequence of the coding region showed 78-82% identity with those of rhodopsins of higher vertebrates, but only 43-47% identity with those of human color pigments. The cloned DNA appears to be the cDNA of a lamprey rhodopsin, which is expressed in the "short" photoreceptor cell.
利用寡核苷酸混合物作为引物，通过聚合酶链反应扩增编码七鳃鳗视觉色素的基因组DNA片段。通过分别扩增cDNA的两端获得了cDNA的完整编码区。编码区推导的氨基酸序列与高等脊椎动物视紫红质的氨基酸序列具有78-82%的同一性，但与人类色觉色素的氨基酸序列仅具有43-47%的同一性。克隆的DNA似乎是七鳃鳗视紫红质的cDNA，其在“短”光感受器细胞中表达。
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