一种新型妊娠特异性β1-糖蛋白成员的克隆与表达

Cloning and expression of a new pregnancy-specific beta 1-glycoprotein member.

作者信息

Plouzek C A, Watanabe S, Chou J Y

机构信息

Human Genetics Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.

出版信息

Biochem Biophys Res Commun. 1991 May 15;176(3):1532-8. doi: 10.1016/0006-291x(91)90461-f.

DOI:10.1016/0006-291x(91)90461-f

PMID:1840485

Abstract

Human pregnancy-specific beta 1-glycoproteins (PSGs) are a group of closely related placental glycoproteins and members of the immunoglobulin superfamily. To study possible functional roles of PSG, three type 1 cDNA clones which encode PSG in a N-A1-A2-B2-C domain arrangement have been characterized in this laboratory. Now, we report the cloning of a type II cDNA (PSG9) which is 1512 bp and encodes a PSG of 332 amino acids in a N-A1-B2-C domain arrangement. In vitro expression experiments demonstrate that PSG9 encodes a 36 kDa nonglycosylated protein which is processed to a glycosylated protein of 46 kDa. Ribonuclease protection analyses show that PSG mRNAs that share sequence similarity with the PSG9 transcript are expressed in both human placenta and placental fibroblasts.

摘要

人妊娠特异性β1-糖蛋白（PSG）是一组密切相关的胎盘糖蛋白，属于免疫球蛋白超家族成员。为了研究PSG可能的功能作用，本实验室已对三个以N-A1-A2-B2-C结构域排列编码PSG的1型cDNA克隆进行了表征。现在，我们报告克隆了一个II型cDNA（PSG9），其长度为1512 bp，以N-A1-B2-C结构域排列编码一个含332个氨基酸的PSG。体外表达实验表明，PSG9编码一种36 kDa的非糖基化蛋白，该蛋白可加工成46 kDa的糖基化蛋白。核糖核酸酶保护分析表明，与PSG9转录本具有序列相似性的PSG mRNA在人胎盘和胎盘成纤维细胞中均有表达。