Izadjoo M J, Mense M G, Bhattacharjee A K, Hadfield T L, Crawford R M, Hoover D L
Department of Environmental and Infectious Disease Sciences, Armed Forces Institute of Pathology, Washington, DC 20306-6000, USA.
Transbound Emerg Dis. 2008 May;55(3-4):145-51. doi: 10.1111/j.1865-1682.2008.01019.x.
To study the safety of Brucella melitensis WR201, a live vaccine candidate, we compared the course of infection of this strain with that of virulent 16M in male BALB/c mice. At various times after oral immunization with strains WR201 or 16M, lungs, liver, spleen, testis, epididymis, inguinal and cervical lymph nodes were removed. Tissues were divided for microbiologic culture and histopathological examination. WR201 infection in male BALB/c mice had lower intensity and shorter duration than infection caused by virulent 16M. Pathological examination of testis and epididymis revealed no inflammation following strain WR201 immunization. In contrast, animals given virulent 16M strain had substantial inflammation in infected tissues. These data confirm the marked attenuation of WR201 relative to 16M. In addition, these studies suggest that male mice may be useful to assess the safety of live, attenuated Brucella vaccine candidates.
为研究布鲁氏菌 melitensis WR201（一种候选活疫苗）的安全性，我们比较了该菌株与强毒株 16M 在雄性 BALB/c 小鼠中的感染过程。在用 WR201 或 16M 菌株口服免疫后的不同时间，取出肺、肝、脾、睾丸、附睾、腹股沟和颈部淋巴结。将组织分开进行微生物培养和组织病理学检查。雄性 BALB/c 小鼠中 WR201 的感染强度低于强毒株 16M，且持续时间更短。睾丸和附睾的病理学检查显示，WR201 菌株免疫后无炎症。相比之下，给予强毒株 16M 的动物在感染组织中有大量炎症。这些数据证实了 WR201 相对于 16M 有明显的减毒。此外，这些研究表明雄性小鼠可能有助于评估减毒活布鲁氏菌候选疫苗的安全性。
