绵羊种布鲁氏菌ΔabcBA基因缺失的活疫苗株对小鼠模型实验性攻毒的保护作用
Protection Provided by an Encapsulated Live Attenuated ΔabcBA Strain of Brucella ovis against Experimental Challenge in a Murine Model.
作者信息
Silva Ana Patrícia C, Macêdo Auricélio A, Silva Teane M A, Ximenes Luana C A, Brandão Humberto M, Paixão Tatiane A, Santos Renato L
机构信息
Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
出版信息
Clin Vaccine Immunol. 2015 Jul;22(7):789-97. doi: 10.1128/CVI.00191-15. Epub 2015 May 6.
Abstract
This study aimed to evaluate the Brucella ovis ΔabcBA strain as a vaccine candidate in the murine model. BALB/c mice were subcutaneously or intraperitoneally immunized with a single dose or three doses of the B. ovis ΔabcBA strain and then were challenged with wild-type B. ovis. Single or multiple immunizations provided only mild protection, with significantly smaller numbers of wild-type B. ovis CFU in the livers of immunized mice but not in the spleens. Encapsulation of B. ovis ΔabcBA significantly improved protection against experimental challenges in both BALB/c and C57BL/6 mice. Furthermore, immunization with encapsulated B. ovis ΔabcBA markedly prevented lesions in the spleens and livers of experimentally challenged mice. These results demonstrated that the encapsulated B. ovis ΔabcBA strain confers protection to mice; therefore, this strain has potential as a vaccine candidate for rams.
摘要
本研究旨在评估绵羊布鲁氏菌ΔabcBA菌株作为小鼠模型中的候选疫苗。将BALB/c小鼠皮下或腹腔注射单剂量或三剂量的绵羊布鲁氏菌ΔabcBA菌株，然后用野生型绵羊布鲁氏菌进行攻毒。单次或多次免疫仅提供轻度保护，免疫小鼠肝脏中的野生型绵羊布鲁氏菌CFU数量显著减少，但脾脏中未减少。绵羊布鲁氏菌ΔabcBA的包封显著提高了对BALB/c和C57BL/6小鼠实验性攻毒的保护作用。此外，用包封的绵羊布鲁氏菌ΔabcBA免疫显著预防了实验性攻毒小鼠脾脏和肝脏中的病变。这些结果表明，包封的绵羊布鲁氏菌ΔabcBA菌株可使小鼠获得保护；因此，该菌株有潜力作为公羊的候选疫苗。
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