酿酒酵母第三条染色体上减数分裂重组热点的遗传分析。
Genetic analysis of a meiotic recombination hotspot on chromosome III of Saccharomyces cerevisiae.
作者信息
Symington L S, Brown A, Oliver S G, Greenwell P, Petes T D
机构信息
Institute of Cancer Research, Columbia University, New York, New York 10032.
出版信息
Genetics. 1991 Aug;128(4):717-27. doi: 10.1093/genetics/128.4.717.
Abstract
In a previous study, we analyzed meiotic recombination events that occurred in the 22-kb region (LEU2 to CEN3) of chromosome III of Saccharomyces cerevisiae. We found one region with an enhanced level of crossovers (a hotspot) and one region with a depressed level of crossovers. In this study, we show that about one-third of the crossovers that occur between LEU2 and CEN3 are initiated in a 1.3-kb region located approximately 6 kb from the centromere. Both crossovers and gene conversion events are initiated at this site. Events initiated at this position can be resolved as crossovers in regions located either centromere-distally or centromere-proximally from the initiation site.
摘要
在之前的一项研究中，我们分析了酿酒酵母第三条染色体上22 kb区域（LEU2至CEN3）发生的减数分裂重组事件。我们发现一个交叉水平增强的区域（一个热点）和一个交叉水平降低的区域。在本研究中，我们表明，发生在LEU2和CEN3之间的交叉事件中约三分之一起始于距着丝粒约6 kb的一个1.3 kb区域。交叉事件和基因转换事件均在此位点起始。在此位置起始的事件可以在起始位点着丝粒远端或近端的区域中解析为交叉事件。
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