Lambie E J, Roeder G S
Department of Biology, Yale University, New Haven, Connecticut 06511.
Cell. 1988 Mar 25;52(6):863-73. doi: 10.1016/0092-8674(88)90428-x.
The centromere of chromosome III (CEN3) of yeast has been examined for its ability to inhibit meiotic recombination in adjacent sequences. The effect of the centromere was investigated when it was adjacent to both of the recombining sequences (homozygous) or adjacent to only one of the two recombining DNA segments (hemizygous). When homozygous, CEN3 exerts a bidirectional repression of crossing over and a strong inhibition of gene conversion. This suggests that CEN3 reduces the frequency of crossing over by interfering with the initiation of proximal recombination events. When hemizygous, CEN3 impairs the ability of adjacent sequences to act as the recipient of genetic information during gene conversion. These results support the idea that the initiating event in yeast meiotic recombination involves the recipient molecule.
人们已对酵母三号染色体（CEN3）的着丝粒抑制相邻序列减数分裂重组的能力进行了研究。当着丝粒与两个重组序列均相邻（纯合）或仅与两个重组DNA片段之一相邻（半合）时，研究了着丝粒的作用。纯合时，CEN3对交换发挥双向抑制作用，并强烈抑制基因转换。这表明CEN3通过干扰近端重组事件的起始来降低交换频率。半合时，CEN3会损害相邻序列在基因转换过程中作为遗传信息受体的能力。这些结果支持了酵母减数分裂重组起始事件涉及受体分子的观点。
