Thammasittirong Anon, Attathom Tipvadee
Department of Science, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen, Nakorn Pathom 73140, Thailand.
J Invertebr Pathol. 2008 Jun;98(2):121-6. doi: 10.1016/j.jip.2008.03.001. Epub 2008 Mar 13.
A total of 134 isolates of Bacillus thuringiensis obtained from different geographical and ecological origins in Thailand were analyzed to determine the distribution and diversity of cry1, cry2 and cry9 genes encoding for Cry proteins toxic to lepidopteran insects. Strains containing cry1-type genes (109/134 or 81.3%) were found at the same frequency as strains harboring cry2 gene (108/134 or 80.6%) whereas only 50 strains contained cry9 gene (50/134 or 37.3%). Seventeen percent (23/134) of the B. thuringiensis isolates did not harbor any cry1, cry2 or cry9 genes. Among cry1 containing isolates, cry1A (49.3%), cry1B (50.0%), cry1G (48.5%), cry1I (49.3%), cry1J (35.1%) and cry1L (47.0%) were considered abundant. The cry2 gene was distributed with high frequency (>70%) in every region of the country. The study of cry gene combinations revealed 14 cry gene profiles.
对从泰国不同地理和生态来源获得的总共134株苏云金芽孢杆菌分离株进行了分析，以确定编码对鳞翅目昆虫有毒的Cry蛋白的cry1、cry2和cry9基因的分布和多样性。含有cry1型基因的菌株（109/134或81.3%）与含有cry2基因的菌株（108/134或80.6%）出现频率相同，而只有50株含有cry9基因（50/134或37.3%）。17%（23/134）的苏云金芽孢杆菌分离株不含有任何cry1、cry2或cry9基因。在含有cry1的分离株中，cry1A（49.3%）、cry1B（50.0%）、cry1G（48.5%）、cry1I（49.3%）、cry1J（35.1%）和cry1L（47.0%）被认为是丰富的。cry2基因在该国每个地区都有高频率分布（>70%）。cry基因组合研究揭示了14种cry基因图谱。
