Shan T, Wang Y, Wu T, Guo J, Liu J, Feng J, Xu Z
Institute of Feed Science, Zhejiang University, The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, No. 164 Qiutao North Road, Hangzhou 310029, PR China.
J Anim Sci. 2008 Aug;86(8):1781-8. doi: 10.2527/jas.2007-0659. Epub 2008 Apr 11.
Adipose triglyceride lipase (ATGL) was recently identified and described as a major novel triglyceride lipase in animals. In this study, we aimed to study the tissue-specific and developmental expression pattern of porcine ATGL (pATGL) and the effect of resveratrol (RES) on expression of pATGL in vitro. The full-length cDNA sequence of pATGL was 1,958 bp (accession no. EF583921), with a 1,458-bp open reading frame encoding a 486-AA protein (the predicted molecular mass of 53.2 kDa, accession no. ABS58651). Comparison of the deduced AA sequence with the bovine, mouse, rat, dog, and human adipose triglyceride lipase showed 87, 84, 83, 81, and 80% similarity, respectively. Furthermore, the pATGL was highly expressed in porcine adipose tissue, to a lesser degree in kidney, heart, and muscle, and least but detectable in brain. In s.c. adipose tissue, pATGL mRNA was low at birth (1 kg of BW) and then increased, reaching a maximal value at 20 kg of BW (approximately 8 wk old; P < 0.01). In peritoneal and omental adipose tissue, the greatest expression of pATGL was observed at 40 kg of BW (approximately 12 wk old). In vitro, exposure of cultured adipocytes to 40 and 80 muM RES for 24 h increased the mRNA levels of pATGL by 95.3% (P < 0.05) and 146.8% (P < 0.01), respectively. Accordingly, lipid accumulation was decreased by 25.7% (P < 0.05) and 60.8% (P < 0.01), respectively. When treated with RES for 48 h, the mRNA levels of pATGL were increased by 104.1% (P < 0.05) and 163.1% (P < 0.01), respectively. As expected, lipid accumulation was decreased by 9.7% (P > 0.05) and 29.0% (P < 0.05), respectively. These results add to our understanding of the role of pATGL in adipose tissue development and as a potential target for regulating fat deposition and meat quality.
脂肪甘油三酯脂肪酶（ATGL）最近被鉴定并描述为动物体内一种主要的新型甘油三酯脂肪酶。在本研究中，我们旨在研究猪ATGL（pATGL）的组织特异性和发育表达模式，以及白藜芦醇（RES）对体外pATGL表达的影响。pATGL的全长cDNA序列为1958 bp（登录号EF583921），有一个1458 bp的开放阅读框，编码一个486个氨基酸的蛋白质（预测分子量为53.2 kDa，登录号ABS58651）。将推导的氨基酸序列与牛、小鼠、大鼠、狗和人的脂肪甘油三酯脂肪酶进行比较，相似性分别为87%、84%、83%、81%和80%。此外，pATGL在猪脂肪组织中高度表达，在肾脏、心脏和肌肉中的表达程度较低，在大脑中表达最少但可检测到。在皮下脂肪组织中，pATGL mRNA在出生时（体重1 kg）较低，然后升高，在体重20 kg（约8周龄；P<0.01）时达到最大值。在腹膜和网膜脂肪组织中，pATGL在体重40 kg（约12周龄）时表达最高。在体外，培养的脂肪细胞暴露于40和80 μM RES 24小时后，pATGL的mRNA水平分别增加了95.3%（P<0.05）和146.8%（P<0.01）。相应地，脂质积累分别减少了25.7%（P<0.05）和60.8%（P<0.01）。当用RES处理48小时时，pATGL的mRNA水平分别增加了104.1%（P<0.05）和163.1%（P<0.01）。正如预期的那样，脂质积累分别减少了9.7%（P>0.05）和29.0%（P<0.05）。这些结果加深了我们对pATGL在脂肪组织发育中的作用以及作为调节脂肪沉积和肉质的潜在靶点的理解。
