Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, 510642, Guangzhou, Guangdong, China.
Mol Biol Rep. 2010 Oct;37(7):3487-93. doi: 10.1007/s11033-009-9941-4. Epub 2009 Nov 29.
Adipose triglyceride lipase (ATGL) is a triglyceride hydrolysis lipase and is generally related to lipid metabolism in animals. The ATGL gene was well studied in mammals, however very less was known in birds that differed significantly with mammals for lipid metabolism. In this study, cloning, mRNA real time and association analysis was performed to characterize the ATGL gene in birds. Results showed that the obtained ATGL gene cDNA of parrot, quail, duck were 1,651 bp (NCBI accession number: GQ221784), 1,557 bp (NCBI accession number: GQ221783) and 1,440 bp each, encoded 481-, 482- and 279-amino acid (AA) peptide, respectively. The parrot ATGL (pATGL) gene was found to predominantly express in breast muscle and leg muscle, and very higher ATGL mRNA level was also found in heart, abdominal fat and subcutaneous fat. The quail ATGL (qATGL) gene was also predominantly expressed in breast muscle and leg muscle, and then to a much lesser degree in heart. The duck ATGL (dATGL) gene was found to predominantly express in subcutaneous fat and abdominal fat, quite higher ATGL mRNA was also found in heart, spleen, breast muscle and leg muscle. Blast analyses indicated the high homology of ATGL and its patatin region, and moreover, and the active serine hydrolase motif ("GASAG" for "GXSXG") and the glycine rich motif ("GCGFLG" for "GXGXXG") were completely conservative among 14 species. Association analyses showed that c.950+24C>A, c.950+45C>G, c.950+73G>A, c.950+83C>T and c.950+128delA of chicken ATGL gene (cATGL) were all significantly or highly significantly with cingulated fat width (CFW) (P < 0.05 or P < 0.01), and c.777-26C>A, c.950+45C>G, c.950+73G>A and c.950+118C>T were all significantly or highly significantly with pH value of breast muscle (BMPH) (P < 0.05).
脂肪甘油三酯脂肪酶（ATGL）是一种甘油三酯水解脂肪酶，通常与动物的脂质代谢有关。ATGL 基因在哺乳动物中得到了很好的研究，然而，鸟类的 ATGL 基因知之甚少，其脂质代谢与哺乳动物有很大的不同。在这项研究中，我们对鸟类的 ATGL 基因进行了克隆、mRNA 实时和关联分析。结果表明，鹦鹉、鹌鹑、鸭的 ATGL 基因 cDNA 分别为 1651bp（NCBI 登录号：GQ221784）、1557bp（NCBI 登录号：GQ221783）和 1440bp，分别编码 481-、482-和 279 个氨基酸（AA）肽。发现鹦鹉 ATGL（pATGL）基因主要在胸肌和腿肌中表达，心脏、腹部脂肪和皮下脂肪中的 ATGL mRNA 水平也很高。鹌鹑 ATGL（qATGL）基因也主要在胸肌和腿肌中表达，然后在心脏中表达程度较低。鸭 ATGL（dATGL）基因主要在皮下脂肪和腹部脂肪中表达，心脏、脾脏、胸肌和腿肌中的 ATGL mRNA 水平也很高。blast 分析表明，ATGL 及其 patatin 区域具有高度同源性，此外，活性丝氨酸水解酶基序（“GASAG”代表“GXSXG”）和富含甘氨酸基序（“GCGFLG”代表“GXGXXG”）在 14 个物种中完全保守。关联分析表明，鸡 ATGL 基因（cATGL）的 c.950+24C>A、c.950+45C>G、c.950+73G>A、c.950+83C>T 和 c.950+128delA 均与颈侧脂肪宽度（CFW）显著或极显著相关（P<0.05 或 P<0.01），c.777-26C>A、c.950+45C>G、c.950+73G>A 和 c.950+118C>T 均与胸肌 pH 值（BMPH）显著或极显著相关（P<0.05）。
